■该研究的目的是评估和比较酸化的磷氟化物(APF)凝胶与含有生姜的草药牙科凝胶的抗菌功效和再矿化潜力,Salvadorapersica,和Cinnamomumzeylanicum。
■Z.officinale的乙醇提取物,S、Persica,制备了C.zeylanicum。用利天青法和琼脂平板划线法测定了这些提取物对变形链球菌和嗜酸乳杆菌的最低抑菌浓度(MIC)和最低杀菌浓度(MBC),分别。配制了草药牙科凝胶,并使用3-(4,5-二甲基噻唑-2-基)-2,5-二苯基四唑溴化物(MTT)测定法评估其细胞毒性。使用琼脂孔扩散法和时间杀灭测定法评估了APF凝胶和草药牙科凝胶的抗菌敏感性。使用立体显微镜评估再矿化潜力。
■草药牙科凝胶显示出更好的抗菌功效,如针对变形链球菌和嗜酸乳杆菌获得的20和21mm的抑制区所示,分别,与APF凝胶获得的11.50和16.50mm抑制区相比。在24和48小时的时间间隔后,草药牙科凝胶也显示出比APF凝胶更好的再矿化潜力,如分别为0.0061和0.0219的统计学显著p值所示。在细胞毒性试验中,在两个研究组的存在下,成纤维细胞显示出100%的生存力.
■我们的研究结果得出结论,草药牙胶是安全无毒的,与常规APF凝胶相比,由于其良好的抗菌作用和再矿化潜力而具有防龋潜力。
■KohliN,HugarSM,HallikerimathS,etal.酸化的磷酸盐氟化物凝胶与含姜的草药牙科凝胶的抗菌效果和再矿化潜力的比较评价,Salvadorapersica,和Cinnamomumzeylanicum:一项体外研究。IntJClinPediatrDent2024;17(3):307-315。
UNASSIGNED: The aim of the study is to evaluate and compare the antibacterial efficacy and remineralization potential of acidulated phosphate fluoride (APF) gel with
herbal dental gel containing Zingiber officinale, Salvadora persica, and Cinnamomum zeylanicum.
UNASSIGNED: Ethanolic extracts of Z. officinale, S. persica, and C. zeylanicum were prepared. Minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of these extracts were determined against Streptococcus mutans and Lactobacillus acidophilus using the resazurin method and agar plate streaking method, respectively. The
herbal dental gel was formulated, and its cytotoxicity was evaluated using an 3-(4,5-dimethythiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assay. The antibacterial susceptibility of APF gel and the
herbal dental gel was assessed using the agar well diffusion method and time-kill assay. The remineralizing potential was evaluated using a stereomicroscope.
UNASSIGNED: Herbal dental gel showed better antibacterial efficacy as depicted by the zone of inhibition of 20 and 21 mm obtained against S. mutans and L. acidophilus, respectively, compared to 11.50 and 16.50 mm zone of inhibition obtained by APF gel. The herbal dental gel also showed better remineralization potential than APF gel after a time interval of 24 and 48 hours, as depicted with a statistically significant p-value of 0.0061 and 0.0219, respectively. In the cytotoxicity test, the fibroblasts showed 100% viability in the presence of both study groups.
UNASSIGNED: The results of our study concluded that
herbal dental gel is safe and nontoxic, having anticariogenic potential due to its good antibacterial action and remineralization potential as compared to conventional APF gel.
UNASSIGNED: Kohli N, Hugar SM, Hallikerimath S, et al. Comparative Evaluation of Antibacterial Efficacy and Remineralization Potential of Acidulated Phosphate Fluoride Gel with Herbal Dental Gel Containing Zingiber officinale, Salvadora persica, and Cinnamomum zeylanicum: An In Vitro Study. Int J Clin Pediatr Dent 2024;17(3):307-315.