Meliponaquadrifascifiataanthidioides和Scaptotrigonadepilis是能够生产蜂胶的无刺蜜蜂,具有相当大的生物勘探潜力。在这种情况下,这项研究的目的是确定化学组成和评价蜂胶生产的抗微生物活性。制备了M.q.anthidioides(EEP-M)和S.depilis(EEP-S)蜂胶的乙醇提取物,用HPLC-ESI-MS对其化学成分进行了表征。对细菌和真菌的抗菌活性进行了评估,从对抗生素作用具有抗性的参考菌株和医院来源分离。从EEP-M,酚类化合物被注释,包括没食子酸,鞣花酸,和类黄酮,以及二萜和三萜。EEP-S在其化学组成中主要显示三萜。两种提取物都能抑制医学相关细菌和真菌的生长,包括医院获得的和抗微生物药物。总的来说,与EEP-M相比,EEP-S显示出更好的抗菌活性。EEP-S对耐万古霉素粪肠球菌的MIC为3.50mg/mL,EEP-M的MIC为5.33±0.16mg/mL。总之,这项研究表明,由M.q.anthioides和S.depilis生产的蜂胶具有预防或治疗微生物感染的潜力。
Melipona quadrifasciata anthidioides and Scaptotrigona depilis are species of stingless bees capable of producing propolis, which has considerable bioprospecting potential. In this context, the objective of this study was to determine the chemical compositions and evaluate the antimicrobial activity of propolis produced by M. q. anthidioides and S. depilis. The ethanolic extracts of propolis of M. q. anthidioides (EEP-M) and S. depilis (EEP-S) were prepared, and their chemical constituents were characterized by HPLC-ESI-MS. The antimicrobial activity was evaluated against bacteria and fungi, isolated from reference strains and hospital origin resistant to the action of antibiotics. From EEP-M, phenolic compounds were annotated, including gallic acid, ellagic acid, and flavonoids, as well as diterpenes and triterpenes. EEP-S showed mainly triterpene in its chemical composition. Both extracts inhibited the growth of medically relevant bacteria and fungi, including hospital-acquired and antimicrobial-resistant. In general, EEP-S showed better antimicrobial activity compared to EEP-M. The MIC of EEP-S against vancomycin-resistant Enterococcus faecalis was 3.50 mg/mL, while the MIC of EEP-M was 5.33 ± 0.16 mg/mL. In conclusion, this study shows that propolis produced by M. q. anthidioides and S. depilis has the potential to be used for the prevention or treatment of microbial infections.