■乙酰辅酶A酰基转移酶-1(ACAA1)是参与脂肪酸代谢的过氧化物酶体酰基转移酶。目前的证据并没有准确揭示ACAA1基因对猪生长性能的影响。
■本研究评估了心脏中ACAA1基因的mRNA表达水平,肝脏,脾,脾肺,6个月大的湘苏猪的肾脏和不同生长阶段的背最长肌(新生,6个月和12个月龄)使用RT-qPCR。采用Sanger测序法对184只健康湘苏猪进行了6月龄和12月龄湘苏猪ACAA1基因单核苷酸多态性(SNPs)与生长性状的关系研究。
■ACAA1基因在心脏中表达,肝脏,脾,脾肺,肾,6个月大的猪的背肌,在肝脏中表达水平最高。ACAA1基因在背最长肌中的表达随年龄增长而下降(p<0.01)。此外,在ACAA1基因中鉴定了四个SNP,包括外显子g.48810A>G(rs343060194),内含子g.51546T>C(rs319197012),外显子g.55035T>C(rs333279910),和外显子g.55088C>T(rs322138947)。对于四个SNP,发现了Hardy-Weinberg平衡(p>0.05),连锁不平衡(LD)分析显示,g.55035T>C(rs333279910)和g.55088C>T(rs322138947)(r2=1.000)之间存在很强的LD。关联分析表明,g.48810A>G(rs343060194),g.51546T>C(rs319197012),g.55035T>C(rs333279910),和g.55088C>T(rs322138947)的体重变化,身体长度,身体高度,腹围,6个月大和12个月大的湘苏猪之间的腿和臀围以及生活脂肪厚度。
■这些发现有力地表明,ACAA1基因可用于标记辅助选择,以改善湘苏猪的生长相关表型,并为分子猪育种提供新的候选基因。
UNASSIGNED: Acetyl-Coenzyme A Acyltransferase-1 (ACAA1) is a peroxisomal acyltransferase involved in fatty acid metabolism. Current evidence does not precisely reveal the effect of the ACAA1 gene on pig growth performance.
UNASSIGNED: The present study assessed the mRNA expression levels of the ACAA1 gene in the heart, liver, spleen, lung, kidney of 6-month-old Xiangsu pigs and in the longissimus dorsi muscle at different growth stages (newborn, 6 months and 12 months of age) using RT-qPCR. The relationship between single-nucleotide polymorphisms (SNPs) of ACAA1 gene and growth traits in 6-month-old and 12-month-old Xiangsu pigs was investigated on 184 healthy Xiangsu pigs using Sanger sequencing.
UNASSIGNED: The ACAA1 gene was expressed in heart, liver, spleen, lung, kidney, and longissimus dorsi muscle of 6-month-old pigs, with the highest level of expression in the liver. ACAA1 gene expression in the longissimus dorsi muscle decreased with age (p < 0.01). In addition, four SNPs were identified in the ACAA1 gene, including exon g.48810 A>G (rs343060194), intron g.51546 T>C (rs319197012), exon g.55035 T>C (rs333279910), and exon g.55088 C>T (rs322138947). Hardy-Weinberg equilibrium (p > 0.05) was found for the four SNPs, and linkage disequilibrium (LD) analysis revealed a strong LD between g.55035 T>C (rs333279910) and g.55088 C>T (rs322138947) (r 2 = 1.000). Association analysis showed that g.48810 A>G (rs343060194), g.51546 T>C (rs319197012), g.55035 T>C (rs333279910), and g.55088 C>T (rs322138947) varied in body weight, body length, body height, abdominal circumference, leg and hip circumference and living backfat thickness between 6-month-old and 12-month-old Xiangsu pigs.
UNASSIGNED: These findings strongly demonstrate that the ACAA1 gene can be exploited for marker-assisted selection to improve growth-related phenotypes in Xiangsu pigs and present new candidate genes for molecular pig breeding.