背景:血小板含有增强组织修复机制的生长因子,包括表皮生长因子(EGF),血小板衍生生长因子(PDGF-AA和-AB),和转化生长因子(TGF)-β。与透明质酸和安慰剂相比,自体富血小板血浆(PRP)已显示出显着改善肌腱损伤的治疗效果。血小板浓度和生长因子之间的协议的主题已经在一些以前的研究中,但生长因子水平与血小板浓度没有很好的相关性.
方法:在本研究中,通过J6-MI离心机浓缩血小板制备自体PRP.采用全自动血液分析仪SysmexXN-20分析PRP中血小板浓度,通过ELISA测定PRP生长因子,包括PDGF,转化生长因子-β1(TGF-β1),和EGF。采用Pearson相关分析对107例自体PRP患者的数据进行统计学分析。
结果:Pearson相关分析显示PDGF,TGF,和EGF与最终PRP产品的血小板浓度具有很强的正相关性(分别为r=0.697,p<0.0001;r=0.488,p<0.0001;r=0.572,p<0.0001)。结论:最终PRP产品中的血小板浓度与PDGF-AB水平之间存在很强的正相关性,TGF-β,和EGF。这些结果表明,直接且具有成本效益的生长因子测试可以提供有关PRP中血小板含量的有价值的信息。
BACKGROUND: Platelet contains growth factors that enhance tissue repair mechanisms, including epidermal growth factor (EGF), platelet-derived growth factor (PDGF-AA and -AB), and transforming growth factor (TGF)-β. Autologous platelet-rich plasma (PRP) has been shown to significantly improve the treatment of tendon injuries compared with hyaluronic acid and placebo. The topic of agreement between platelet concentrations and growth factors has been covered in some previous studies, but growth factor levels did not correlate well with platelet concentrations.
METHODS: In this study, autologous PRP was prepared by concentrating platelets through a J6-MI centrifuge. The automatic hematology analyzer Sysmex XN-20 was used to analyze the platelet concentration in PRP, and the PRP growth factors were determined by ELISA, including PDGF, transforming growth factor- β1 (TGF-β1), and EGF. Statistical analysis was conducted on data from 107 patients who received autologous PRP using Pearson correlation analysis.
RESULTS: Pearson correlation analysis revealed PDGF, TGF, and EGF had a strong positive correlation with the platelet concentration of the final PRP product (r = 0.697, p < 0.0001; r = 0.488, p < 0.0001; r = 0.572, p < 0.0001, respectively) CONCLUSIONS: There was a strong positive correlation between the concentration of platelets in the final PRP product and the levels of PDGF-AB, TGF-β, and EGF. These results suggested straightforward and cost-effective growth factor tests can provide valuable information about platelet content in PRP.