近年来,越来越多的兴趣集中在野生动植物在生态学和抗微生物耐药性(AMR)进化中的贡献上。这项研究的目的是通过分子研究在马尔切地区(意大利中部)发现死亡的金jack狼(Canisaureus)的器官样品中抗菌抗性基因(ARG)的存在。肺样本,肝脏,脾,脾肾,和肠道通过靶向以下基因的PCR进行研究:tet(A),tet(B),tet(C),tet(D),tet(E),tet(G),tet(K),tet(L),tet(M),tet(O),tet(S),tet(P),tet(Q),tet(X),sul1,sul2,sul3,blaCTX-M,blaSHV,blaTEM,和mcr-1到mcr-10。在所有测试的器官中检测到一种或多种ARGs,除了脾脏.具体来说,肺和肝脏tet(M)和tet(P)阳性,肾脏为mcr-1,肠道为tet(A),tet(L),tet(M),tet(O),tet(P),sul3和blaTEM-1。这些结果,根据狼的机会主义觅食策略,确认其作为AMR环境污染的良好生物指示剂的潜在作用。
In recent years an increasing interest has been focused on the contribution of wildlife in ecology and evolution of the antimicrobial resistance (AMR). The aim of this study was to molecularly investigate the presence of antimicrobial resistance genes (ARGs) in organ samples from a golden jackal (Canis aureus) found dead in the Marche region (Central Italy). Samples from lung, liver, spleen, kidney, and intestine were investigated by PCRs targeting the following genes: tet(A), tet(B), tet(C), tet(D), tet(E), tet(G), tet(K), tet(L), tet(M), tet(O), tet(S), tet(P), tet(Q), tet(X), sul1, sul2, sul3, blaCTX-M, blaSHV, blaTEM, and mcr-1 to mcr-10. One or more ARGs were detected in all organs tested, except the spleen. Specifically, the lung and liver were positive for tet(M) and tet(P), the kidney for mcr-1 and the intestine for tet(A), tet(L), tet(M), tet(O), tet(P), sul3 and blaTEM-1. These results, according to the opportunistic foraging strategy of the jackal, confirm its potential role as a good bioindicator of AMR environmental contamination.