Abstract：
Forty-six golden jackals (Canis aureus) were collected between November 2020 and February 2021 in five counties of Serbia. Lung samples were screened for the presence of Pneumocystis DNA by pan-Pneumocystis PCR on the mtLSU rRNA gene obtaining PCR products of 370 bp in length. Pneumocystis DNA was detected in the lungs from 6/46 (13.04%) golden jackals. Four were females and two were males; four were classified as adults and two as subadults. Positive samples were confirmed in 4/5 investigated counties. No gross pathologic lung lesions were observed in this study. The sequences of Pneumocystis spp. from golden jackals were identical to one another and showed the highest similarity with Pneumocystis spp. sequences of dogs (98% nucleotide identity). The genetic variation was comparable to Pneumocystis spp. of raccoon dogs (95-97% nucleotide identity), red foxes (91-95% nucleotide identity), ferrets (86% nucleotide identity), and another Pneumocystis type in dogs (P. canis Ck2, 81% nucleotide identity) was higher. Golden jackals may be carriers and may play a nonnegligible role in the spread of Pneumocystis spp. Although this finding cannot be directly related to any clinical manifestation or pathologic lesions, a possible role in the exacerbation of different pulmonary disorders should be considered.
摘要：
在2020年11月至2021年2月之间，在塞尔维亚的五个县收集了46头金狼（Canisaureus）。通过对mtLSUrRNA基因进行泛-肺孢子虫PCR，筛选肺样品中是否存在肺孢子虫DNA，获得长度为370bp的PCR产物。在6/46(13.04%)金狼的肺中检测到肺孢子虫DNA。四个是女性，两个是男性；四个被归类为成年人，两个被归类为亚成年人。在4/5调查的县中确认了阳性样本。在这项研究中没有观察到大体病理性肺部病变。肺孢子虫的序列。金jack狼彼此相同，与肺孢子虫属的相似性最高。狗的序列(98%核苷酸同一性)。遗传变异与肺孢子虫属相当。浣熊狗（95-97％的核苷酸同一性），赤狐（91-95%核苷酸同一性），雪貂（86%核苷酸同一性），和狗的另一种肺孢子虫类型(P.犬Ck2，81%核苷酸同一性)较高。金jack狼可能是携带者，并且在肺孢子虫的传播中起着不可忽视的作用。尽管这一发现与任何临床表现或病理损害没有直接关系，应考虑在不同肺部疾病恶化中的可能作用.
