巨型病毒,归类为核细胞病毒群,被认为普遍存在于自然环境中。然而,关于孤立的巨型病毒的全面报告仍然很少,关于不可恢复菌株的信息有限,病毒增殖位点,自然宿主以前,作者强调了潘多拉病毒,潘多拉病毒,和Mimivirussp.styx,从微咸水土壤中分离出来,作为巨型病毒繁殖的潜在热点。这项研究提供了在分离出上述三种菌株后,在同一微咸水区域内每月采样近一年的结果。本报告详细介绍了各种巨型病毒的反复分离。每个月,从大约5×10米的地块随机收集了四个土壤样本,包括三个土壤样品和一个含有河床沉积物的水样。castellanii棘阿米巴被用作病毒分离的宿主。这些努力持续每月至少产生一种病毒,总共有55个巨型病毒分离株。最常见的分离物种是Mimiviridae(24个分离株),其次是Marseilleviridae(23个分离株),潘多拉病毒(6株),以及Pithovirus和Cedratvirus的单一分离株。值得注意的是,每个月从四个样本中的任何一个样本中都没有分离出病毒,某些位点不产生病毒。基于分离株数量的聚类分析显示,5月的土壤样品以及1月的水和沉积物样品产生的病毒株数量最高。这些发现强调了咸淡的沿海土壤是分离许多巨型病毒的重要场所。突出了海岸线上的非均匀分布。
Giant viruses, categorized under Nucleocytoviricota, are believed to exist ubiquitously in natural environments. However, comprehensive reports on isolated giant viruses remain scarce, with limited information available on unrecoverable strains, viral proliferation sites, and natural hosts. Previously, the author highlighted Pandoravirus hades, Pandoravirus persephone, and Mimivirus sp. styx, isolated from brackish water soil, as potential hotspots for giant virus multiplication. This study presents findings from nearly a year of monthly sampling within the same brackish water region after isolating the three aforementioned strains. This report details the recurrent isolation of a wide range of giant viruses. Each month, four soil samples were randomly collected from an approximately 5 × 10 m plot, comprising three soil samples and one water sample containing sediment from the riverbed. Acanthamoeba castellanii was used as a host for virus isolation. These efforts consistently yielded at least one viral species per month, culminating in a total of 55 giant virus isolates. The most frequently isolated species was Mimiviridae (24 isolates), followed by Marseilleviridae (23 isolates), Pandoravirus (6 isolates), and singular isolates of Pithovirus and Cedratvirus. Notably, viruses were not consistently isolated from any of the four samples every month, with certain sites yielding no viruses. Cluster analysis based on isolate numbers revealed that soil samples from May and water and sediment samples from January produced the highest number of viral strains. These findings underscore brackish coastal soil as a significant site for isolating numerous giant viruses, highlighting the non-uniform distribution along coastlines.