PDF(Pubmed)
Abstract：
The discovery of mimivirus in 2003 prompted the search for novel giant viruses worldwide. Despite increasing interest, the diversity and distribution of giant viruses is barely known. Here, we present data from a 2012-2022 study aimed at prospecting for amoebal viruses in water, soil, mud, and sewage samples across Brazilian biomes, using Acanthamoeba castellanii for isolation. A total of 881 aliquots from 187 samples covering terrestrial and marine Brazilian biomes were processed. Electron microscopy and PCR were used to identify the obtained isolates. Sixty-seven amoebal viruses were isolated, including mimiviruses, marseilleviruses, pandoraviruses, cedratviruses, and yaraviruses. Viruses were isolated from all tested sample types and almost all biomes. In comparison to other similar studies, our work isolated a substantial number of Marseillevirus and cedratvirus representatives. Taken together, our results used a combination of isolation techniques with microscopy, PCR, and sequencing and put highlight on richness of giant virus present in different terrestrial and marine Brazilian biomes.
摘要：
2003年发现的模仿病毒促使人们在全球范围内寻找新型巨型病毒。尽管兴趣越来越大,巨型病毒的多样性和分布鲜为人知。这里,我们提供了2012-2022年研究的数据，旨在寻找水中的变形虫病毒，土壤,泥浆,巴西生物群落的污水样本，使用卡斯特拉尼棘阿米巴进行隔离。总共处理了来自187个样本的881个等分试样，这些样本涵盖了巴西陆地和海洋生物群落。使用电子显微镜和PCR鉴定获得的分离物。分离出67种变形虫病毒,包括模仿病毒，马赛病毒,泛病毒,cedratviruses,和yaravirus。从所有测试的样品类型和几乎所有的生物群落中分离病毒。与其他类似研究相比，我们的工作分离出了大量的马赛病毒和柏树病毒代表。一起来看,我们的结果使用了分离技术与显微镜的结合，PCR,并进行测序，并重点介绍了巴西不同陆地和海洋生物群落中存在的巨型病毒的丰富度。
