Abstract：
OILTS is a viral class I terpene synthase found from the giant virus Orpheovirus IHUMI-LCC2. It exhibits a unique structure and demonstrates high plasticity to metal cofactors, allowing it to biosynthesize different cyclic terpene frameworks. Notably, while OILTS produces only (+)-germacrene D-4-ol with the most common cofactor, Mg2+, it also biosynthesizes a different cyclic terpene, (+)-cubebol, with Mn2+, Co2+, or Ni2+, presenting a rare instance of cofactor-dependent enzyme catalysis. This is the first report of (+)-cubebol biosynthesis, to our knowledge. In addition, OILTS can uptake Zn2+ as a cofactor, which is uncommon among ordinary terpene synthases. These findings suggest that OILTS\'s functional plasticity may benefit the virus in diverse host environments, highlighting potential evolutionary implications.
摘要：
OILTS是从巨大病毒OrphovirusIHUMI-LCC2中发现的I类病毒萜烯合酶。它具有独特的结构，对金属辅因子具有高可塑性，允许它生物合成不同的环状萜烯框架。值得注意的是,虽然油仅产生具有最常见辅因子的(+)-雌二醇D-4-醇，Mg2+,它还生物合成了不同的环状萜烯，(+)-cubebol,与Mn2+，Co2+,或Ni2+，呈现一种罕见的辅因子依赖性酶催化。这是（+）-cubebol生物合成的第一份报告，我们的知识。此外,油可以吸收Zn2+作为辅因子，这在普通萜合酶中并不常见。这些发现表明，OILTS的功能可塑性可能使病毒在不同的宿主环境中受益，强调潜在的进化影响。
