背景:伯氏杆菌是一种新发现的细菌,与猪的肺炎关系不明确。然而,很少有研究充分描述了这个物种。
结果:在这项研究中,我们分析了形态学,生理,和新鉴定的芽孢杆菌的基因组特征。11月。从猪中分离出的菌株QD2021。B.porcorumQD2021菌株的完整基因组序列由单个环形染色体(2,271,736bp,38.51%G+C含量),编码2578个基因。检测到一个大小为70,040bp的质粒。共有121个分散的重复序列,319个串联重复序列,4个基因组岛,5个预言,3个CRISPR序列,并预测了51个ncRNAs。在八个数据库中成功地注释了芽孢杆菌基因组的编码基因(NR,GO,KEGG,COG,TCDB,普法姆,Swiss-Prot和CAZy)和四个致病性相关数据库(PHI,卡,VFDB和ARDB)。此外,进行了比较基因组分析,以探索B.porcorumQD2021的进化关系。
结论:据我们所知,这是为B.porcorum提供基本表型和全基因组序列的第一项研究。我们的结果广泛扩展了当前的知识,并可以作为未来研究的宝贵基因组资源。
BACKGROUND: Bergeyella porcorum is a newly identified bacterium that has an ambiguous relationship with pneumonia in pigs. However, few studies have adequately characterized this species.
RESULTS: In this study, we analyzed the morphological, physiological, and genomic characteristics of the newly identified B. porcorum sp. nov. strain QD2021 isolated from pigs. The complete genome sequence of the B. porcorum QD2021 strain consists of a single circular chromosome (2,271,736 bp, 38.51% G + C content), which encodes 2,578 genes. One plasmid with a size of 70,040 bp was detected. A total of 121 scattered repeat sequences, 319 tandem repeat sequences, 4 genomic islands, 5 prophages, 3 CRISPR sequences, and 51 ncRNAs were predicted. The coding genes of the B. porcorum genome were successfully annotated across eight databases (NR, GO, KEGG, COG, TCDB, Pfam, Swiss-Prot and CAZy) and four pathogenicity-related databases (PHI, CARD, VFDB and ARDB). In addition, a comparative genome analysis was performed to explore the evolutionary relationships of B. porcorum QD2021.
CONCLUSIONS: To our knowledge, this is the first study to provide fundamental phenotypic and whole-genome sequences for B. porcorum. Our results extensively expand the current knowledge and could serve as a valuable genomic resource for future research on B. porcorum.