在Sprague-Dawley大鼠中评估了通过代谢工程化谷氨酸棒杆菌发酵产生的口服L-色氨酸的亚慢性毒性。将0、500、1000和2000mg/kg/天的剂量给予10只雄性和10只雌性大鼠的组90天。对于0和2000mg/kg/天的组，另外5只雄性和5只雌性大鼠作为恢复组进行测试。在90天的产品给药期间，在所有大鼠中均未观察到与测试物质相关的不良反应，无论剂量如何，并在0和2000mg/kg/天的剂量下恢复4周。此外，L-色氨酸相关的嗜酸性粒细胞增多-肌痛综合征(EMS)的组织化学和免疫组织化学分析未显示0或2000mg/kg/天给药组的两种性别均有显著变化.基于这些结果,可以得出结论，在所有动物中都没有与测试物质有关的明显不良反应；因此，干燥的L-色氨酸发酵产物可用作饲料添加剂材料。
The subchronic toxicity of oral L-tryptophan produced by fermentation with metabolically engineered Corynebacterium glutamicum was evaluated in Sprague-Dawley rats. Doses of 0, 500, 1000, and 2000 mg/kg/day were administered to groups of 10 male and 10 female rats for 90 days. For the groups administered 0 and 2000 mg/kg/day, an additional 5 male and 5 female rats were tested as a recovery group. No adverse effects associated with the test substance were observed in all rats during the 90-day administration of the product, irrespective of dose, and at 4 weeks of recovery at dosages of 0 and 2000 mg/kg/day. Furthermore, histochemical and immunohistochemical analyses for L-tryptophan-associated eosinophilia-myalgia syndrome (EMS) did not reveal significant changes in both sexes of groups administered 0 or 2000 mg/kg/day. Based on these results, it could be concluded that there were no significant adverse effects related to the test substance in all animals; therefore, dried L-tryptophan fermentation product can be used as feed additive material.
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