背景:幽门螺杆菌感染是胃肠道疾病的重要病原体。先前的研究已经确定单核苷酸多态性(SNP)是与幽门螺杆菌感染相关的因素。值得注意的是,Leb和唾液酸-Lex抗原,受FUT3和FUT6基因调控,在幽门螺杆菌感染中起着至关重要的作用。本研究旨在探讨中国北方汉族人群FUT3和FUT6基因多态性与幽门螺杆菌感染的相关性。
方法:采用免疫比浊法检测幽门螺杆菌感染,将受试者分为感染组和未感染组。通过测序鉴定基因变体。最后,分析FUT3和FUT6基因多态性以评估其与幽门螺杆菌感染的相关性。
结果:感染组中T等位基因(rs778805)和G等位基因(rs61147939)的频率明显高于非感染组(63.4%vs.55.1%,p=0.045;55.2%vs.47.0%,分别为p=0.042)。在感染组中,隐性模型中AA基因型(rs3745635)的频率,隐性模型中的TT基因型(rs778805),和GG基因型(rs61147939)在隐性模型显著高于非感染组(5.8%vs.2.3%,p=0.042;41.9%vs.29.3%,p=0.022;34.9%vs.20.5%,分别为p=0.0068)。感染组FUT6基因的A13单倍型和A13/A13复型的频率明显高于非感染组(55.56%vs.46.32%,p=0.019;34.94%vs.20.30%,分别为p=0.045)。rs778805-rs17855739-rs28362459-rs3745635组合被鉴定为最佳相互作用模型(p<0.05)。
结论:这项研究表明,在中国北方的汉族人群中,FUT3和FUT6基因多态性与幽门螺杆菌感染显著相关。
BACKGROUND: Helicobacter pylori infection is a significant pathogen in gastrointestinal diseases. Previous studies have identified single-nucleotide polymorphisms (SNPs) are factors associated with H. pylori infection. Notably, Leb and Sialyl-Lex antigens, regulated by the FUT3 and FUT6 genes, play a crucial role in H. pylori infection. This study aimed to investigate the correlation between FUT3 and FUT6 gene polymorphisms and H. pylori infection in the Han population of northern China.
METHODS: An immunoturbidimetric assay was employed to detect H. pylori infection, categorizing subjects into infected and noninfected groups. Gene variants were identified through sequencing. Finally, FUT3 and FUT6 gene polymorphisms were analyzed to assess their association with H. pylori infection.
RESULTS: The frequency of the T allele (rs778805) and the G allele (rs61147939) in the infection group was significantly higher than that in the noninfection group (63.4% vs. 55.1%, p = 0.045; 55.2% vs. 47.0%, p = 0.042, respectively). In the infection group, the frequency of the AA genotype (rs3745635) in the recessive model, the TT genotype (rs778805) in the recessive model, and the GG genotype (rs61147939) in the recessive model were significantly higher than the noninfection group (5.8% vs. 2.3%, p = 0.042; 41.9% vs. 29.3%, p = 0.022; 34.9% vs. 20.5%, p = 0.0068, respectively). The frequency of the A13 haplotype and the A13/A13 diplotype of the FUT6 gene was significantly higher in the infection group than in the noninfection group (55.56% vs. 46.32%, p = 0.019; 34.94% vs. 20.30%, p = 0.045, respectively). The rs778805-rs17855739-rs28362459-rs3745635 combination was identified as the best interaction model (p < 0.05).
CONCLUSIONS: This study suggests that FUT3 and FUT6 gene polymorphisms are significantly associated with H. pylori infection in the Han Chinese from northern China.