Abstract：
Different methods for fixing biofilms of Staphylococcus aureus and Salmonella enterica for light and electron microscopy were compared. Paraformaldehyde fixation did not preserve biofilm integrity during dehydration; Ito-Karnovsky fixation revealed cell morphology, but did not preserve the matrix. Ruthenium red combined with aldehydes allowed the matrix to be preserved and visualized. An analysis of the ultrastructure of S. aureus and S. enterica cells in biofilms and suspensions at various fixations is presented. The ultrastructure of the biofilm matrix has been described.
摘要：
比较了用光镜和电子显微镜固定金黄色葡萄球菌和沙门氏菌生物膜的不同方法。多聚甲醛固定在脱水过程中不能保持生物膜的完整性；伊藤-卡诺夫斯基固定显示细胞形态，但没有保留矩阵。钌红与醛结合允许基质被保存和可视化。介绍了在各种固定状态下生物膜和悬浮液中金黄色葡萄球菌和肠葡萄球菌细胞的超微结构分析。已经描述了生物膜基质的超微结构。
