多柔比星(DOX)是一种重要的肿瘤治疗药物,但是由于其毒性和副作用,其临床应用受到限制。因此,在治疗过程中检测DOX的浓度对于提高疗效和减少副作用至关重要。在这项研究中,作者开发了一种基于局部表面等离子体共振(LSPR)的生物光子光纤传感器,该传感器具有基于多模光纤(MMF)-四芯光纤(FCF)-七芯光纤(SCF)-MMF的直接锥形和反锥形结构,用于特定的DOX检测。与其他检测方法相比,它具有灵敏度高的优点,低成本,抗干扰能力强。在这个实验中,多壁碳纳米管(MWCNT),氧化铈纳米棒(CeO2-NRs),和金纳米粒子(AuNPs)固定在探针表面以增强传感器的生物相容性。MWCNT和CeO2-NRs为AuNP的固定提供了更多的结合位点。通过在表面上固定AuNPs,消逝场刺激LSPR以检测DOX。传感器表面用DOX适体功能化以进行特异性检测,增强其特异性。实验表明,在0-10µM的线性检测范围内,传感器的灵敏度为0.77nm/µM,检测限(LoD)为0.42µM。此外,探头的可重复性,再现性,稳定性,和选择性进行了评估,表明该探针在癌症治疗期间具有检测DOX的高潜力。
Doxorubicin (DOX) is an important drug for cancer treatment, but its clinical application is limited due to its toxicity and side effects. Therefore, detecting the concentration of DOX during treatment is crucial for enhancing efficacy and reducing side effects. In this study, the authors developed a biophotonic fiber sensor based on localized surface plasmon resonance (LSPR) with the multimode fiber (MMF)-four core fiber (FCF)-seven core fiber (SCF)-MMF-based direct-taper and anti-taper structures for the specific detection of DOX. Compared to other detection methods, it has the advantages of high sensitivity, low cost, and strong anti-interference ability. In this experiment, multi-walled carbon nanotubes (MWCNTs), cerium-oxide nanorods (CeO2-NRs), and gold nanoparticles (AuNPs) were immobilized on the probe surface to enhance the sensor\'s biocompatibility. MWCNTs and CeO2-NRs provided more binding sites for the fixation of AuNPs. By immobilizing AuNPs on the surface, the LSPR was stimulated by the evanescent field to detect DOX. The sensor surface was functionalized with DOX aptamers for specific detection, enhancing its specificity. The experiments demonstrated that within a linear detection range of 0-10 µM, the sensitivity of the sensor is 0.77 nm/µM, and the limit of detection (LoD) is 0.42 µM. Additionally, the probe\'s repeatability, reproducibility, stability, and selectivity were evaluated, indicating that the probe has high potential for detecting DOX during cancer treatment.