分析了长期弹药污染对微生态特性的影响,制定了微生物修复策略。具体来说,酶系统的反应,N/O稳定同位素,离子网络,和微生物群落结构/功能水平在长期(50年)弹药污染的水/沉积物从污染地点进行了分析,并选择了一种能够有效降解三硝基甲苯(TNT)同时耐受许多重金属的复合细菌剂来修复弹药污染的土壤。水/沉积物的基本物理和化学性质(pH(最高:0.57-0.64),硝酸盐(上升:1.31-4.28倍),亚硝酸盐(上升:1.51-5.03倍),和铵(上升:7.06-70.93倍)变化明显,N和O(硝酸盐氮)的稳定同位素比的显着差异证实了长期暴露于污染的本地微生物对TNT的降解能力。重金属,如Pb,Zn,Cu,Cd,Cs,Sb,在弹药污染的场所有协同毒性作用,并显著降低了核心污染区的微生物多样性和丰富度。然而,长期暴露在边缘污染区诱导微生物利用TNT作为碳源和氮源进行生命活动和生长发育。拟杆菌微生物群受到弹药污染的显著抑制,而微生物如变形杆菌,酸杆菌,和Com科通过调节其发育和应激反应逐渐适应这种环境胁迫。弹药污染显著影响微生态遗传网络中的DNA复制和基因调控,增加了人类健康风险。Mg和K显著参与了微生物运输的内在机制,富集,和TNT的代谢。筛选了9株利用TNT的微生物,它们具有有效的TNT降解和对典型重金属的耐受性(铜,锌和铅)在受污染的地方发现,为有效修复弹药污染土壤而制备的复合细菌剂显著改善了土壤生态环境。
The effects of long-term ammunition pollution on microecological characteristics were analyzed to formulate microbial remediation strategies. Specifically, the response of enzyme systems, N/O stable isotopes, ion networks, and microbial community structure/function levels were analyzed in long-term (50 years) ammunition-contaminated water/sediments from a contamination site, and a compound bacterial agent capable of efficiently degrading trinitrotoluene (TNT) while tolerating many heavy metals was selected to remediate the ammunition-contaminated soil. The basic physical and chemical properties of the water/sediment (pH (up: 0.57-0.64), nitrate (up: 1.31-4.28 times), nitrite (up: 1.51-5.03 times), and ammonium (up: 7.06-70.93 times)) were changed significantly, and the significant differences in stable isotope ratios of N and O (nitrate nitrogen) confirmed the degradability of TNT by indigenous microorganisms exposed to long-term pollution. Heavy metals, such as Pb, Zn, Cu, Cd, Cs, and Sb, have synergistic toxic effects in ammunition-contaminated sites, and significantly decreased the microbial diversity and richness in the core pollution area. However, long-term exposure in the edge pollution area induced microorganisms to use TNT as a carbon and nitrogen sources for life activities and growth and development. The Bacteroidales microbial group was significantly inhibited by ammunition contamination, whereas microorganisms such as Proteobacteria, Acidobacteriota, and Comamonadaceae gradually adapted to this environmental stress by regulating their development and stress responses. Ammunition pollution significantly affected DNA replication and gene regulation in the microecological genetic networks and increased the risk to human health. Mg and K were significantly involved in the internal mechanism of microbial transport, enrichment, and metabolism of TNT. Nine strains of TNT-utilizing microbes were screened for efficient TNT degradation and tolerance to typical heavy metals (copper, zinc and lead) found in contaminated sites, and a compound bacterial agent prepared for effective repair of ammunition-contaminated soil significantly improved the soil ecological environment.