■肥胖,一个公共健康问题,是一种影响慢性退行性疾病发展的炎症状态,特别是严重肥胖患者。
■这项研究的目的是证明不同程度肥胖患者的免疫代谢差异,包括严重的肥胖,通过确定淋巴细胞亚群和代谢之间的相关性,身体成分,和临床变量。
■外周血免疫细胞(CD4+,CD8+记忆和效应T淋巴细胞)进行分析,和身体成分的测量,血压,和生化成分(葡萄糖,糖化血红蛋白(HbA1c),胰岛素,C反应蛋白(CRP),和血脂谱)在不同程度的肥胖患者中进行。
■根据全身脂肪(TBF)百分比将患者分类为正常体脂,1级和2级肥胖,3类肥胖,和4级肥胖。TBF百分比越大,身体组成(如定义为肌少症肥胖的无脂肪量(FFM)的减少)和免疫代谢谱的差异越明显.CD3+T淋巴细胞增加(主要是CD4+,CD4+CD62-,和CD8+CD45RO+T淋巴细胞)和TBF百分比的增加(肥胖的严重程度)。
■淋巴细胞亚群与代谢,身体成分,临床变量证明了慢性疾病的存在,肥胖的低强度炎症过程。因此,通过淋巴细胞亚群测定重度肥胖患者的免疫代谢谱有助于确定疾病的严重程度和肥胖相关慢性退行性疾病的风险增加.
Obesity, a public health problem, is a state of metainflammation that influences the development of chronic degenerative diseases, particularly in patients with severe obesity.
The objective of this study was to evidence immunometabolic differences in patients with different degrees of obesity, including severe obesity, by determining correlations between lymphocyte subpopulations and metabolic, body composition, and clinical variables.
Peripheral blood immune cells (CD4+, CD8+ memory and effector T lymphocytes) were analyzed, and measures of body composition, blood pressure, and biochemical composition (glucose, glycated hemoglobin (HbA1c), insulin, C-reactive protein (CRP), and the lipid profile) were carried out in patients with different degrees of obesity.
The patients were classified according to total body fat (TBF) percentage as normal body fat, class 1 and 2 obesity, class 3 obesity, and class 4 obesity. The greater the TBF percentage, the more pronounced the differences in body composition (such as a decrease in the fat-free mass (FFM) that is defined as sarcopenic obesity) and the immunometabolic profile. There was an increase of CD3+ T lymphocytes (mainly CD4+, CD4+CD62-, and CD8+CD45RO+ T lymphocytes) and an increase in the TBF percentage (severity of obesity).
The correlations between lymphocyte subpopulations and metabolic, body composition, and clinical variables demonstrated the existence of a chronic, low-intensity inflammatory process in obesity. Therefore, measuring the immunometabolic profile by means of lymphocyte subpopulations in patients with severe obesity could be useful to determine the severity of the disease and the increased risk of presenting obesity-associated chronic degenerative diseases.