METHODS: We divided endometriosis patients into high and low groups based on the expression levels of VISTA in lesion tissues. We collected peritoneal fluid samples from these two groups and performed 16 s RNA sequencing and metabolomics analysis to investigate microbial diversity and differential metabolites. Through combined analysis, we identified microbial-associated metabolites and validated their correlation with VISTA and CD8 + T cells using ELISA and immunofluorescence. In vitro experiments were conducted to confirm the regulatory relationship among these factors.
RESULTS: Our findings revealed a distinct correlation between VISTA expression and the microbial colony Escherichia.Shigella. Moreover, we identified the metabolites LTD4-d5 and 2-n-Propylthiazolidine-4-carboxylic acid as being associated with both Escherichia.Shigella and VISTA expression. In vitro experiments confirmed the inhibitory effects of these metabolites on VISTA expression, while they demonstrated a positive regulation of CD8 + T cell infiltration into endometriotic lesions.
CONCLUSIONS: This study reveals the connection between microbial diversity, metabolites, and VISTA expression in the immune microenvironment of endometriosis, providing potential targets for therapeutic interventions.
方法:我们根据病灶组织中VISTA的表达水平将子宫内膜异位症患者分为高和低两组。我们收集了这两组的腹膜液样本,并进行了16sRNA测序和代谢组学分析,以研究微生物多样性和差异代谢物。通过组合分析,我们鉴定了微生物相关代谢物,并使用ELISA和免疫荧光验证了它们与VISTA和CD8+T细胞的相关性.进行体外实验以确认这些因素之间的调节关系。
结果:我们的发现揭示了VISTA表达与埃希氏菌菌落之间的明显相关性。志贺氏菌.此外,我们确定了代谢物LTD4-d5和2-n-丙基噻唑烷-4-羧酸与两种埃希氏菌有关。志贺氏菌和VISTA表达。体外实验证实了这些代谢物对VISTA表达的抑制作用,同时他们表现出CD8+T细胞浸润到子宫内膜异位病变的正调控。
结论:这项研究揭示了微生物多样性之间的联系,代谢物,和VISTA在子宫内膜异位症免疫微环境中的表达,为治疗干预提供潜在的目标。