UNASSIGNED: Here, we developed an IFN-γ release assay (IGRA) that mainly detects JCV-specific effector memory T cells and effectors T cells in the blood.
UNASSIGNED: This assay was frequently positive in patients with active PML (with a positive JCV PCR in CSF) of various underlying immunosuppression causes (84% sensitivity). Only 3% of healthy donors had a positive response (97% specificity). The frequency of positivity also increased in multiple sclerosis patients according to the time on natalizumab (up to 36% in patients treated for more than 48 months, who are considered at a higher risk of PML).
UNASSIGNED: The results show this assay\'s frequent or increased positivity in patients with PML or an increased risk of PML, respectively. The assay may help to stratify the risk of PML.
■这里,我们开发了一种IFN-γ释放试验(IGRA),主要检测血液中JCV特异性效应记忆T细胞和效应T细胞。
■该测定在具有各种潜在免疫抑制原因的活动性PML(在CSF中具有阳性JCVPCR)的患者中通常为阳性(84%的灵敏度)。只有3%的健康供体具有阳性反应(97%特异性)。根据使用那他珠单抗的时间,多发性硬化症患者的阳性频率也增加了(治疗超过48个月的患者高达36%,被认为患PML的风险较高)。
■结果显示,在患有PML或PML的风险增加的患者中,该测定频繁或增加阳性,分别。该测定可能有助于对PML的风险进行分层。