背景:肠道菌群显著影响红斑石斑鱼(Epinephelusakaara)的健康和生长,一种来自中国南方福建省的著名商业海鱼。然而,生存策略和季节的变化会影响肠道微生物群数据的稳定性,使其在反映肠道微生物群的状态时不准确。通过对肠道微生物群的细致理解,这阻碍了水产养殖健康的有效增强。受此启发,我们在四个季节对野生和圈养的E.akaara的肠道微生物群进行了全面分析。
结果:从东山市的野生和圈养种群中收集了72个E.akaara样本,在四个不同的季节。收集肠道的四个部分以获得有关肠道微生物组成的全面信息,并使用16SrRNA下一代IlluminaMiSeq进行测序。在冬季,我们观察到圈养和野生E.akaara的肠道微生物多样性最高。并使用Mantel分析确定了与水温的强相关性。与野生E.akaara相比,我们在圈养的E.akaara中发现了一个更复杂的微生物网络,芽孢杆菌科的丰度增加证明了这一点,斑马鱼科和肠杆菌科。相比之下,弧菌科,梭菌科,发现黄杆菌科和红杆菌科在野生E.akaara中更有活性。然而,一些核心微生物,如Firmicutes和光细菌,在野生组和圈养组中都显示出相似的分布模式。此外,我们发现了阿卡拉从前肠到后肠的前10个核心微生物的常见群落组成和分布特征。
结论:总的来说,该研究提供了对E.akaara肠道微生物群的相对更全面的描述,考虑到生存策略和时间维度,这对E.akaara的肠道微生物群产生了有价值的见解,并为其水产养殖提供了有价值的参考。
BACKGROUND: The gut microbiota significantly influences the health and growth of red-spotted grouper (Epinephelus akaara), a well-known commercial marine fish from Fujian Province in southern China. However, variations in survival strategies and seasons can impact the stability of gut microbiota data, rendering it inaccurate in reflecting the state of gut microbiota. Which impedes the effective enhancement of aquaculture health through a nuanced understanding of gut microbiota. Inspired by this, we conducted a comprehensive analysis of the gut microbiota of wild and captive E. akaara in four seasons.
RESULTS: Seventy-two E. akaara samples were collected from wild and captive populations in Dongshan city, during four different seasons. Four sections of the gut were collected to obtain comprehensive information on the gut microbial composition and sequenced using 16S rRNA next-generation Illumina MiSeq. We observed the highest gut microbial
diversity in both captive and wild E. akaara during the winter season, and identified strong correlations with water temperature using Mantel analysis. Compared to wild E. akaara, we found a more complex microbial network in captive E. akaara, as evidenced by increased abundance of Bacillaceae, Moraxellaceae and Enterobacteriaceae. In contrast, Vibrionaceae, Clostridiaceae, Flavobacteriaceae and Rhodobacteraceae were found to be more active in wild E. akaara. However, some core microorganisms, such as Firmicutes and Photobacterium, showed similar distribution patterns in both wild and captive groups. Moreover, we found the common community composition and distribution characteristics of top 10 core microbes from foregut to hindgut in E. akaara.
CONCLUSIONS: Collectively, the study provides relatively more comprehensive description of the gut microbiota in E. akaara, taking into account survival strategies and temporal dimensions, which yields valuable insights into the gut microbiota of E. akaara and provides a valuable reference to its aquaculture.