PDF(Pubmed)
Abstract：
BACKGROUND: Lichens, traditionally considered as a simple partnership primarily between mycobiont and photobiont, are, in reality, complex holobionts comprised of a multitude of microorganisms. Lichen mycobiome represents fungal community residing within lichen thalli. While it is acknowledged that factors like the host lichen species and environmental conditions influence the structure of the lichen mycobiome, the existing research remains insufficient. To investigate which factor, host genus or location, has a greater impact on the lichen mycobiome, we conducted a comparative analysis of mycobiomes within Parmelia and Peltigera collected from both Turkey and South Korea, using high-throughput sequencing based on internal transcribed spacer region amplification.
RESULTS: Overall, the lichen mycobiome was dominated by Capnodiales (Dothideomycetes), regardless of host or location. At the order level, the taxonomic composition was not significantly different according to lichen genus host or geographical distance. Hierarchical clustering of the top 100 abundant ASVs did not clearly indicate whether the lichen mycobiome was more influenced by host genus or location. Analyses of community similarity and partitioning variables revealed that the structure of the lichen mycobiome is more significantly influenced by location than by host genus. When analyzing the core mycobiome by host genus, the Peltigera mycobiome contained more ASV members than the Parmelia mycobiome. These two core mycobiomes also share common fungal strains, including basidiomycete yeast. Additionally, we used chi-squared tests to identify host genus-specialists and location-specialists.
CONCLUSIONS: By comparing lichen mycobiomes of the same genera across different countries, our study advances our comprehension of these microbial communities. Our study elucidates that, although host species play a contributory role, geographic distance exerts a more pronounced impact on the structure of lichen mycobiome. We have made foundational contributions to understanding the lichen mycobiome occupying ecologically crucial niches. We anticipate that broader global-scale investigations into the fungal community structures will provide more detailed insights into fungal residents within lichens.
RESULTS: Overall, the lichen mycobiome was dominated by Capnodiales (Dothideomycetes), regardless of host or location. At the order level, the taxonomic composition was not significantly different according to lichen genus host or geographical distance. Hierarchical clustering of the top 100 abundant ASVs did not clearly indicate whether the lichen mycobiome was more influenced by host genus or location. Analyses of community similarity and partitioning variables revealed that the structure of the lichen mycobiome is more significantly influenced by location than by host genus. When analyzing the core mycobiome by host genus, the Peltigera mycobiome contained more ASV members than the Parmelia mycobiome. These two core mycobiomes also share common fungal strains, including basidiomycete yeast. Additionally, we used chi-squared tests to identify host genus-specialists and location-specialists.
CONCLUSIONS: By comparing lichen mycobiomes of the same genera across different countries, our study advances our comprehension of these microbial communities. Our study elucidates that, although host species play a contributory role, geographic distance exerts a more pronounced impact on the structure of lichen mycobiome. We have made foundational contributions to understanding the lichen mycobiome occupying ecologically crucial niches. We anticipate that broader global-scale investigations into the fungal community structures will provide more detailed insights into fungal residents within lichens.
摘要：
背景：地衣，传统上被认为是一种简单的伙伴关系，主要是在真菌和光生物之间，是，在现实中,由多种微生物组成的复杂的完整生物。地衣真菌群代表地衣内的真菌群落。虽然人们承认寄主地衣种类和环境条件等因素会影响地衣真菌生物群落的结构，现有的研究仍然不足。为了调查哪个因素，宿主属或位置，对地衣真菌群的影响更大，我们对从土耳其和韩国收集的Parmelia和Peltigera中的分枝杆菌进行了比较分析，使用基于内部转录间隔区扩增的高通量测序。
结果：总体而言，地衣真菌群系以Capnodiales（Dothideomycetes）为主，无论主机或位置。在订单层面,根据地衣属寄主或地理距离，分类组成没有显着差异。前100名丰富的ASV的分层聚类并未清楚地表明地衣真菌群是否受宿主属或位置的影响更大。对群落相似性和分配变量的分析表明，地衣真菌群的结构受位置的影响比受寄主属的影响更大。当按宿主属分析核心真菌生物群落时，Peltigera真菌群比Parmelia真菌群包含更多的ASV成员。这两个核心真菌也有共同的真菌菌株，包括担子菌酵母.此外,我们使用卡方检验来识别宿主属专家和定位专家。
结论：通过比较不同国家相同属的地衣真菌，我们的研究提高了我们对这些微生物群落的理解。我们的研究阐明了，尽管寄主物种起着重要的作用，地理距离对地衣真菌群的结构产生了更明显的影响。我们为了解占据生态关键生态位的地衣真菌生物群做出了基础性贡献。我们预计，对真菌群落结构的更广泛的全球调查将为地衣内的真菌居民提供更详细的见解。
结果：总体而言，地衣真菌群系以Capnodiales（Dothideomycetes）为主，无论主机或位置。在订单层面,根据地衣属寄主或地理距离，分类组成没有显着差异。前100名丰富的ASV的分层聚类并未清楚地表明地衣真菌群是否受宿主属或位置的影响更大。对群落相似性和分配变量的分析表明，地衣真菌群的结构受位置的影响比受寄主属的影响更大。当按宿主属分析核心真菌生物群落时，Peltigera真菌群比Parmelia真菌群包含更多的ASV成员。这两个核心真菌也有共同的真菌菌株，包括担子菌酵母.此外,我们使用卡方检验来识别宿主属专家和定位专家。
结论：通过比较不同国家相同属的地衣真菌，我们的研究提高了我们对这些微生物群落的理解。我们的研究阐明了，尽管寄主物种起着重要的作用，地理距离对地衣真菌群的结构产生了更明显的影响。我们为了解占据生态关键生态位的地衣真菌生物群做出了基础性贡献。我们预计，对真菌群落结构的更广泛的全球调查将为地衣内的真菌居民提供更详细的见解。
