背景:ADIPOQ基因rs822393变异体对饮食干预减肥后胰岛素抵抗和脂联素水平等代谢参数的影响仍不确定。这项研究的目的是评估ADIPOQ基因的rs822393在12周内以地中海模式的高脂肪低热量饮食减肥后对脂联素水平和代谢参数的作用。
方法:将283名肥胖患者分配到一项为期12周的高脂肪低热量饮食的饮食干预试验中。确定了脂肪和生化参数。rs822393采用显性模型分析(CCvsCT+TT)进行评估。结果这些患者有三种不同的基因型:CC(59.0%),CT(33.6%),和TT(7.4%)。C和T的等位基因频率分别为0.89和0.20。基础和干预后HDL胆固醇,T等位基因携带者的脂联素水平和脂联素/瘦素比低于非T等位基因携带者。饮食干预后,BMI,体重,脂肪量,腰围,收缩压,胰岛素,HOMA-IR,瘦素,两个基因型组的总胆固醇和LDL-胆固醇水平均显著改善.此外,HDL-胆固醇(CCvsCT+TT)(delta:8.9+1.1mg/dlvs.1.7+0.8毫克/分升;p=0.02),非T等位基因携带者的血清脂联素(43.1±5.9ng/dlvs.2.8±3.0ng/dl;p=0.01)和脂联素/瘦素比率(1.37±0.1单位与0.17±0.08单位;p=0.02)仅在体重减轻后的非T等位基因携带者中有所改善。
结论:肥胖且无rs822393T等位基因的个体脂联素水平改善,遵循地中海模式的高脂肪低热量饮食后的脂联素/瘦素比率和HDL-胆固醇水平。
BACKGROUND: The effects of the rs822393 variant of ADIPOQ gene on metabolic parameters such as insulin resistance and adiponectin levels following weight loss through dietary intervention are still uncertain. The aim of this study was to evaluate the role of rs822393 of ADIPOQ gene on adiponectin levels and metabolic parameters after weight loss with a high-fat hypocaloric diet with Mediterranean pattern during 12 weeks.
METHODS: A population of 283 patients with obesity was allocated to a dietary intervention trial with a high-fat hypocaloric diet during 12 weeks. Adiposity and biochemical parameters were determined. rs822393 was assessed with a dominant model analysis (CC vs. CT + TT).
RESULTS: These patients had three different genotypes: CC (59.0%), CT (33.6%), and TT (7.4%). The allelic frequencies for C and T were 0.89 and 0.20, respectively. Basal and post-intervention HDL cholesterol, adiponectin levels, and adiponectin/leptin ratio were lower in T-allele than non-T-allele carriers. After dietary intervention, BMI, weight, fat mass, waist circumference, systolic blood pressure, insulin, HOMA-IR, leptin, total cholesterol, and LDL cholesterol levels improved significantly in both genotype groups. Moreover, HDL cholesterol (CC vs. CT + TT) (delta: 8.9 ± 1.1 mg/dL vs. 1.7 ± 0.8 mg/dL; p = 0.02), serum adiponectin in non-T-allele carriers (43.1 ± 5.9 ng/dL vs. 2.8 ± 3 0.0 ng/dL; p = 0.01), and adiponectin/leptin ratio (1.37 ± 0.1 units vs. 0.17 ± 0.08 units; p = 0.02) improved only in non-T-allele carriers after weight loss.
CONCLUSIONS: Individuals with obesity and without the T allele of rs822393 experienced improvements in adiponectin levels, adiponectin/leptin ratio, and HDL cholesterol levels after following a high-fat hypocaloric diet with a Mediterranean pattern.