一种具有抗菌活性的新型放线菌,指定菌株H16431T,是从滇池收集的沉积物样本中分离出来的,云南省,公关中国。基于16SrRNA基因序列的系统发育分析表明,菌株H16431T与根瘤菌CGMCC4.7431T和广生物学菌CGMCC4.7101T的亲缘关系最密切(相似度为98.1%),但与NonomuraaeaceibaeKCTC39826T形成了单系进化枝(98.0%的相似性)。基于全基因组序列的系统基因组分析表明,菌株H16431T在Nonomuraea属中形成了一个单独的进化枝。平均核苷酸同一性,平均氨基酸同一性,H16431T菌株与其密切相关的Nonomuraea种之间的数字DNA-DNA杂交值为80.0-81.5%,71.2-74.6%,和23.2-25.0%,分别,显着低于广泛接受的物种定义阈值。基于全基因组序列,DNAG+C含量为70.2%。甲萘醌被鉴定为MK-9(H4),MK-9(H6),和MK-9(H2)。主要脂肪酸是异C16:0,10甲基-C17:0和异C16:02OH。磷脂是二磷脂酰甘油,磷脂酰甘油,磷脂酰乙醇胺,羟基磷脂酰乙醇胺,和磷脂酰肌醇。这些化学分类学特征与Nonomuraea属的化学分类学特征相对应。根据分类证据,菌株H16431T代表Nonomuraea属的新物种,命名为Nonomuraeaseedminissp。11月。是提议的。菌株类型为H16431T(=JCM34852T=CICC25119T)。
A novel actinobacterium with antimicrobial activity, designated strain H16431T, was isolated from a sediment sample collected from Dianchi Lake, Yunnan Province, PR China. Phylogenetic analysis based on 16S rRNA gene sequence indicated that strain H16431T was most closely related to Nonomuraea rhizosphaerae CGMCC 4.7431T and Nonomuraea guangzhouensis CGMCC 4.7101T (98.1% similarity), but formed a monophyletic clade with Nonomuraea ceibae KCTC 39826T (98.0% similarity). Phylogenomic analysis based on whole-genome sequence showed that strain H16431T formed a separate clade within the genus Nonomuraea. The average nucleotide identity, average amino acid identity, and digital DNA-DNA hybridization values between strain H16431T and its closely related Nonomuraea species were 80.0-81.5%, 71.2-74.6%, and 23.2-25.0%, respectively, which were significantly lower than the widely accepted species-defined threshold. The DNA G + C content was 70.2% based on the whole-genome sequence. The menaquinones were identified as MK-9(H4), MK-9(H6), and MK-9(H2). The major fatty acids were iso-C16:0, 10 methyl-C17:0, and iso-C16:0 2OH. The phospholipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, hydroxy-phosphatidylethanolamine, and phosphatidylinositol. These chemotaxonomic characteristics were corresponded to those of the genus Nonomuraea. On the basis of the taxonomic evidence, strain H16431T represents a novel species of the genus Nonomuraea, for which the name Nonomuraea sediminis sp. nov. is proposed. The type strain is H16431T (=JCM 34852T=CICC 25119T).