背景:石斛Sw.代表兰科最广阔的属之一,以其物种高药用和观赏价值而闻名。在高等植物中,锚蛋白(ANK)重复蛋白家族的特征是一个独特的ANK重复结构域,不可或缺的生物功能和生化活动。ANK基因家族在植物的各种生理过程中起着举足轻重的作用,包括应激反应,激素信号,和增长。因此,研究ANK基因家族和鉴定石斛抗病基因至关重要。
结果:这项研究鉴定了金村石斛和米戈石斛中的78个ANK基因,77在金黄石斛Lindl。,和58在铁皮石斛Lindl中。随后,我们对这些ANK基因家族进行了全面的生物信息学分析,包括基因分类,染色体定位,系统发育关系,基因结构和基序表征,顺式作用调控元件识别,共线性评估,蛋白质-蛋白质相互作用网络的构建,和基因表达谱分析。同时,3个DoANK基因(DoANK14,DoANK19和DoANK47)通过SA间接激活ETI系统中的NPR1转录因子,从而调节抗菌PR基因的表达。用GA3和ABA的激素处理显示17和8个基因显著上调,而4个和8个基因显著下调,分别。发现DoANK32位于胞吞途径中的ArfGAP基因,影响囊泡运输和生长素的极地运动。
结论:我们的发现为分类学分类提供了一个可靠的框架,进化分析,石斛ANK基因的功能预测。来自D.officinale的三个突出的ANK基因(DoANK14,DoANK19和DoANK47)可能在抗病性和应激反应研究中被证明是有价值的。DoANK32通过其在囊泡运输和生长素极性中的作用与D.officinale的形态发生和发育有关,亚细胞定位研究证实其存在于细胞核和细胞膜中。ANK基因对激素治疗的反应表现出显著的表达变化,可能在D.officinale的激素反应中起着至关重要的作用。通过调节GA3和ABA等植物激素可能抑制其生长和发育。
BACKGROUND: Dendrobium Sw. represents one of the most expansive genera within the Orchidaceae family, renowned for its species\' high medicinal and ornamental value. In higher plants, the ankyrin (ANK) repeat protein family is characterized by a unique ANK repeat domain, integral to a plethora of biological functions and biochemical activities. The ANK gene family plays a pivotal role in various plant physiological processes, including stress responses, hormone signaling, and growth. Hence, investigating the ANK gene family and identifying disease-resistance genes in
Dendrobium is of paramount importance.
RESULTS: This research identified 78 ANK genes in
Dendrobium officinale Kimura et Migo, 77 in
Dendrobium nobile Lindl., and 58 in
Dendrobium chrysotoxum Lindl. Subsequently, we conducted comprehensive bioinformatics analyses on these ANK gene families, encompassing gene classification, chromosomal localization, phylogenetic relationships, gene structure and motif characterization, cis-acting regulatory element identification, collinearity assessment, protein-protein interaction network construction, and gene expression profiling. Concurrently, three DoANK genes (DoANK14, DoANK19, and DoANK47) in D. officinale were discerned to indirectly activate the NPR1 transcription factor in the ETI system via SA, thereby modulating the expression of the antibacterial PR gene. Hormonal treatments with GA3 and ABA revealed that 17 and 8 genes were significantly up-regulated, while 4 and 8 genes were significantly down-regulated, respectively. DoANK32 was found to localize to the ArfGAP gene in the endocytosis pathway, impacting vesicle transport and the polar movement of auxin.
CONCLUSIONS: Our findings provide a robust framework for the taxonomic classification, evolutionary analysis, and functional prediction of
Dendrobium ANK genes. The three highlighted ANK genes (DoANK14, DoANK19, and DoANK47) from D. officinale may prove valuable in disease resistance and stress response research. DoANK32 is implicated in the morphogenesis and development of D. officinale through its role in vesicular transport and auxin polarity, with subcellular localization studies confirming its presence in the nucleus and cell membrane. ANK genes displaying significant expression changes in response to hormonal treatments could play a crucial role in the hormonal response of D. officinale, potentially inhibiting its growth and development through the modulation of plant hormones such as GA3 and ABA.