目的:母亲提供的因素的变化会严重损害产后早期发育,导致她的后代更容易患高血压。TGR(mRen-2)27(TGR)母亲,以过度激活的肾素-血管紧张素系统为特征,在他们的母乳中表现出改变的离子成分。因此,我们旨在分析交叉培养对高血压TGR和血压正常的汉诺威Sprague-Dawley(HanSD)后代心血管参数的影响。
方法:我们通过遥测测量了5至10周龄雄性后代的心血管参数。通过从6至12周龄雄性后代获得的主动脉样品中的蛋白质印迹评估与血管功能相关的蛋白质的表达。通过放射免疫分析(RIA)评估血浆肾素活性和血浆血管紧张素II(AngII)水平。
结果:在TGR中,高血压的发展伴随着低高频比的增加(LF/HF;交感迷走神经平衡的标志;第10周为0.51±0.16)。此外,与HanSD后代相比,TGR表现出盐皮质激素受体(MR;p<0.05)和转化生长因子β1型(TGF-β1;p=0.002)的主动脉表达增加。培养显著降低交感迷走神经平衡(第10周0.23±0.10),暂时,HanSD母亲饲养的TGR后代血浆AngII水平和MR表达。
结论:这些发现强调了理解早期生活经历之间复杂相互作用的重要性。母性因素,以及后来的心血管功能。了解观察到的效果背后的机制可能有助于确定潜在的干预措施,以防止以后生活中高血压的发展。
OBJECTIVE: Early postnatal development can be significantly compromised by changes in factors provided by the mother, leading to increased vulnerability to hypertension in her offspring. TGR(mRen-2)27 (TGR) mothers, characterised by an overactivated renin-angiotensin system, exhibit altered ion composition in their breast milk. Therefore, we aimed to analyse the impact of cross-fostering on cardiovascular parameters in hypertensive TGR and normotensive Hannover Sprague-Dawley (HanSD) offspring.
METHODS: We measured cardiovascular parameters in 5- to 10-week-old male offspring by telemetry. The expression of proteins related to vascular function was assessed by western blotting in the aortic samples obtained from 6- to 12-week-old male offspring. Plasma renin activity and plasma angiotensin II (Ang II) levels were evaluated by radioimmunoassay (RIA).
RESULTS: The development of hypertension was in TGR accompanied by increased low-to-high frequency ratio (LF/HF; a marker of sympathovagal balance; 0.51 ± 0.16 in week 10). Furthermore, TGR exhibited increased aortic expression of mineralocorticoid receptor (MR; p < 0.05) and transforming growth factor beta type 1 (TGF-β1; p = 0.002) compared to HanSD offspring. Fostering significantly decreased sympathovagal balance (0.23 ± 0.10 in week 10) and, transiently, plasma Ang II levels and MR expression in TGR offspring reared by HanSD mothers.
CONCLUSIONS: These findings highlight the importance of understanding the complex interplay between early life experiences, maternal factors, and later cardiovascular function. Understanding the mechanisms behind the observed effects may help to identify potential interventions to prevent the development of hypertension later in life.