目的:角膜成纤维细胞参与角膜的伤口愈合并增殖,迁移,和分化过程。辅酶Q10(CoQ10)和维生素E可以在角膜病变后作为滴眼剂使用时增强角膜伤口的愈合。因此,进行这项研究是为了确定含有CoQ10和维生素ED-α-生育酚聚乙二醇1000琥珀酸酯(TPGS)衍生制剂的CoQ10眼科溶液在体外人角膜成纤维细胞(HCFs)中的潜在效率.方法:从尸体角膜组织中获得原发性HCFs,在24和72小时使用MTT测定法测定细胞活力。使用体外伤口愈合测定法评估细胞迁移,和I型胶原(COL-I)的mRNA表达,III型胶原(COL-III),Lumican,透明质酸,基质金属蛋白酶(MMP)-1,MMP-2,MMP-9,MMP组织抑制剂(TIMP)-1,TIMP-2,白细胞介素(IL)-1β,IL-6,IL-8和IL-10使用逆转录聚合酶链反应在24和72小时进行评估。结果:在各种浓度的CoQ10眼科溶液(CoQ10-os),与对照组相比,HCFs的细胞活力和伤口愈合率增加。COL-I的表达式,COL-III,Lumican,和透明质酸增加了CoQ10-os,而MMP-1,MMP-2,MMP-9,TIMP-1,TIMP-2和TIMP-3的那些在24和72小时均不受CoQ10-os的影响。在用CoQ10-os培养基处理HCFs时,IL-1β,IL-6和IL-8下降,而IL-10以时间和剂量依赖性方式显著增加。结论:研究结果表明,CoQ10和维生素E-TPGS是HCFs生物活性的有效调节剂,从而支持其作为眼科解决方案在旨在快速再生受损角膜组织的治疗中的潜在应用。
Purpose: Corneal fibroblasts are involved in the wound healing of the cornea with proliferation, migration, and differentiation processes. Coenzyme Q10 (
CoQ10) and vitamin E can enhance corneal wound healing when applied after a corneal lesion as an eye drop. Thus, this study was performed to determine the potential efficiency of a
CoQ10 ophthalmical solution containing a CoQ10 and vitamin E D-α-tocopherol polyethylene glycol 1000 succinate (TPGS)-derived formulation in human corneal fibroblasts (HCFs) in vitro. Methods: Primary HCFs were obtained from cadaveric corneal tissue, and cell viability was determined using MTT assay at 24 and 72 h. Cell migration was evaluated using an in vitro wound healing assay, and mRNA expressions of collagen type I (COL-I), collagen type III (COL-III), lumican, hyaluronan, matrix metalloproteinase (MMP)-1, MMP-2, MMP-9, tissue inhibitors of MMP (TIMP)-1, TIMP-2, interleukin (IL)-1β, IL-6, IL-8, and IL-10 were assessed using reverse transcription polymerase chain reaction at 24 and 72 h. Results: At various concentrations of
CoQ10 ophthalmical solution (
CoQ10-os), cell viability and wound healing rates of HCFs increased compared with the control group. The expressions of COL-I, COL-III, lumican, and hyaluronan were increased by
CoQ10-os, whereas those of MMP-1, MMP-2, MMP-9, TIMP-1, TIMP-2, and TIMP-3 were not affected by
CoQ10-os at 24 and 72 h. In treating HCFs with a CoQ10-os medium, IL-1β, IL-6, and IL-8 decreased, whereas IL-10 was significantly increased in a time- and dose-dependent manner. Conclusions: The findings indicate that CoQ10 and vitamin E-TPGS are potent regulators of the bioactivity of HCFs, thus supporting their potential application as ophthalmical solutions in therapies aimed at the fast regeneration of damaged cornea tissues.