Abstract：
One of the factors responsible for less pregnancy rates is the use of frozen semen in sheep due to the oxidative stress created by the process. The aim of this experiment was to test the effects of adding coenzyme Q-10 (CoQ10) to the seminal extender on sperm quality and the pregnancy rate of sheep. In this study, ejaculates from eight Dorper rams of reproductive age were used and tested in four treatments: Control (pure BotuBov®), C1 (175 µM of CoQ10), C3 (350 µM of CoQ10), and C7 (700 µM of CoQ10). Samples were collected in triplicate from each animal, and sperm analysis was performed by CASA after thawing at 0 h and 2 h. The samples were also analyzed by flow cytometry for plasma and acrosomal membrane integrity, stability, lipid peroxidation, mitochondrial potential, and superoxide anion production. In total, 198 ewes were inseminated by laparoscopy and divided into two groups: control (n=98) and C7 (n=100). Pregnancy diagnosis was performed at 30 days. Coenzyme Q10 proved to be safe for semen cryopreservation, not altering sperm kinetic values between the groups post-thawing. In flow cytometry, the C1 and C7 groups achieved a better index of plasma membrane integrity and membrane stability (P<0.05). A increased pregnancy rate was observed in C7 (52 %) compared to the control (38 %). In conclusion, coenzyme Q10 assists in the cryopreservation process, protecting the sperm cell and improving pregnancy rates in ewes.
摘要：
导致怀孕率较低的因素之一是由于该过程产生的氧化应激而在绵羊中使用冷冻精液。本实验的目的是研究在扩精剂中添加辅酶Q-10（CoQ10）对绵羊精子质量和妊娠率的影响。在这项研究中,使用来自八只生育年龄的Dorper公羊的射精并在四种治疗中进行了测试：对照（纯BotuBov®），C1（175µM的CoQ10），C3（350µM的CoQ10），和C7（700µM的CoQ10）。从每只动物中收集样品一式三份，在0h和2h解冻后通过CASA进行精子分析。还通过流式细胞术分析样品的血浆和顶体膜完整性，稳定性,脂质过氧化，线粒体电位，和超氧阴离子生产。总的来说,通过腹腔镜检查对198只母羊进行授精，分为两组：对照组（n=98）和C7（n=100）。在30天进行妊娠诊断。辅酶Q10被证明对精液冷冻保存是安全的，解冻后不会改变各组之间的精子动力学值。在流式细胞术中,C1和C7组取得了较好的质膜完整性和膜稳定性指数(P<0.05)。与对照（38％）相比，C7（52％）的妊娠率增加。总之,辅酶Q10有助于冷冻保存过程，保护精子细胞，提高母羊的妊娠率。
