背景:中风是全球范围内死亡和残疾的重要原因,在死因中排名第二,它被认为与遗传因素有关。我们的研究目的是调查中国人群中CASZ1,WNT2B和PTPRG单核苷酸多态性(SNP)与中风风险之间的关系。
方法:我们招募了1418名志愿者,本研究包括710例中风病例和708例对照。我们使用MassARRAYiPLEXGOLD方法对CASZ1,WNT2B和PTPRG上的三个SNP进行了基因型。使用Logistic回归分析这些SNP与卒中之间的关联。然后计算比值比(ORs)和95%置信区间(CIs)。更重要的是,通过多因素降维(MDR)分析预测SNP之间的相互作用。
结果:这项研究表明,CASZ1rs880315和PTPRGrs704341与卒中易感性降低相关。更确切地说,CASZ1rs880315与年龄≤64岁人群和女性的卒中易感性降低相关。PTPRGrs704341与年龄>64岁人群的卒中易感性降低相关,女人,不吸烟者和不饮酒者。相反,WNT2Brs12037987与年龄>64岁人群的卒中易感性升高有关,女性和非吸烟者。此外,CASZ1rs880315、WNT2Brs12037987和PTPRGrs704341存在较强的冗余关系。
结论:我们的研究得出结论,CASZ1rs880315,WNT2Brs12037987和PTPRGrs704341与中风有关,该研究为评估中国汉族人群中与卒中风险相关的遗传变异提供了基础。
BACKGROUND: Stroke is an important cause of death and disability worldwide, ranking second in the cause of death, and it is thought to be related to genetic factors. The purpose of our study is to investigate the association between CASZ1, WNT2B and PTPRG single nucleotide polymorphisms (SNPs) and stroke risk in the Chinese population.
METHODS: We recruited 1418 volunteers, comprised of 710 stroke cases and 708 controls in this study. We used MassARRAY iPLEX GOLD method to genotype the three SNPs on CASZ1, WNT2B and PTPRG. Logistic regression was used to analyse the association between these SNPs and stroke, and odds ratios (ORs) and 95% confidence intervals (CIs) were then calculated. What\'s more, the interactions among SNPs were predicted by multi-factor dimensionality reduction (MDR) analysis.
RESULTS: This research demonstrated that CASZ1 rs880315 and PTPRG rs704341 were associated with reduced stroke susceptibility. More precisely, CASZ1 rs880315 was associated with reduced stroke susceptibility in people aged ≤64 years and women. PTPRG rs704341 was associated with reduced stroke susceptibility in people aged >64 years, women, non-smokers and non-drinkers. Conversely, WNT2B rs12037987 was related to elevated stroke susceptibility in people aged >64 years, women and non-smokers. In addition, CASZ1 rs880315, WNT2B rs12037987 and PTPRG rs704341 had a strong redundancy relationship.
CONCLUSIONS: Our study concludes that CASZ1 rs880315, WNT2B rs12037987 and PTPRG rs704341 are associated with stroke, and the study provides a basis for assessing genetic variants associated with stroke risk in the Han Chinese population.