目的:比较无龋齿(CF)和有龋齿(CA)个体的口腔微生物群,在分类和功能层面。
方法:本系统综述遵循PRISMA指南进行。在MEDLINE/PUBMED中进行了结构化搜索,WebofScience,EMBASE,LILACS,SciELO,截至9月,Scopus和GoogleScholar数据库,2023年。观察性研究,在不限制发表日期和使用下一代靶向或非靶向测序方法鉴定微生物群落的情况下,纳入研究.对所有纳入的研究进行定性合成。
结果:纳入了54项研究(43项横断面研究;11项研究),包括3486名以上的参与者(至少1666CF和1820CA),他们的唾液和/或牙菌斑被用作临床样本。大多数研究的方法学质量被评为“一般”。CF和CA个体中87个细菌和44个真菌属的丰度具有统计学差异。Atobobiumspp。,Capnocytophagaspp.,乳杆菌属。,普雷沃氏菌属。,Scardoviaspp.,硒单胞菌属。其中经常报告CA个体中更丰富.几个功能模式,如脂质,碳水化合物,淀粉,蔗糖,氨基糖代谢,其中,被确定为与CF或CA条件特别相关。
结论:尽管所包括的研究之间存在差异,并且定性合成占主导地位,微生物群以及由评估的微生物群编码的特定功能谱在受龋齿影响的个体和无龋齿的个体中不同地丰富。考虑到每个评估的主要研究固有的局限性,这些结果需要谨慎解释。
OBJECTIVE: To compare the oral microbiota among caries-free (CF) with caries-affected (CA) individuals, both at taxonomic and at functional levels.
METHODS: This systematic review was conducted following PRISMA guidelines. A structured search was carried out in MEDLINE/PUBMED, Web of Science, EMBASE, LILACS, SciELO, Scopus and Google Scholar databases up to September, 2023. Observational studies, without any restriction on date of publication and using next-generation targeted or untargeted sequencing methods for identification of microbial communities were included. Qualitative synthesis was performed from all included studies.
RESULTS: 54 studies were included (43 cross-sectional; 11 cohort) comprising more than 3486 participants (at least 1666 CF and 1820 CA) whose saliva and/or dental plaque were used as clinical samples. Methodological quality was graded as \"fair\" for most of the studies. The abundance of 87 bacterial and 44 fungal genera were statistically different among CF and CA individuals. Atopobium spp.,
Capnocytophaga spp., Lactobacillus spp., Prevotella spp., Scardovia spp., Selenomonas spp. among others were frequently reported as being more abundant in CA individuals. Several functional patterns, such as lipids, carbohydrate, starch, sucrose, amino sugar metabolisms, among others, were identified as being specifically related to CF or to CA conditions.
CONCLUSIONS: In spite of the variability among the included studies and of the predominance of qualitative synthesis, groups of microorganisms as well as specific functional profiles coded by the assessed microbiota are differently abundant among caries-affected and caries-free individuals. These results need to be interpreted with caution considering the limitations inherent to each assessed primary study.