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Abstract：
BACKGROUND: The aim of the present study was to evaluate the subgingival microbiome in patients with grade C molar-incisor pattern periodontitis (C-MIP) affecting the primary or permanent dentitions.
METHODS: DNA was isolated from subgingival biofilm samples from diseased and healthy sites from 45 C-MIP patients and subjected to phylogenetic microarray analysis. C-MIP sites were compared between children affected in the primary to those affected in the permanent dentitions. Within-subject differences between C-MIP-affected sites and dentition-matched healthy sites were also evaluated.
RESULTS: C-MIP sites of subjects affected in the primary dentition showed partially overlapping but distinct microbial communities from C-MIP permanent dentition sites (p < 0.05). Differences were due to increased levels in primary C-MIP sites of certain species of the genera Capnocytophaga and Leptotrichia, while C-MIP permanent dentition sites showed higher prevalence of Filifactor alocis. Aggregatibacter actinomycetemcomitans (Aa) was among species seen in high prevalence and levels in both primary and permanent C-MIP sites. Moreover, both permanent and primary C-MIP sites showed distinct microbial communities when compared to dentition-matched healthy sites in the same subject (p < 0.01).
CONCLUSIONS: Primary and permanent teeth with C-MIP showed a dysbiotic microbiome, with children affected in the primary dentition showing a distinct profile from those affected in the permanent dentition. However, Aa was enriched in both primary and permanent diseased sites, confirming that this microorganism is implicated in C-MIP in both dentitions.
METHODS: DNA was isolated from subgingival biofilm samples from diseased and healthy sites from 45 C-MIP patients and subjected to phylogenetic microarray analysis. C-MIP sites were compared between children affected in the primary to those affected in the permanent dentitions. Within-subject differences between C-MIP-affected sites and dentition-matched healthy sites were also evaluated.
RESULTS: C-MIP sites of subjects affected in the primary dentition showed partially overlapping but distinct microbial communities from C-MIP permanent dentition sites (p < 0.05). Differences were due to increased levels in primary C-MIP sites of certain species of the genera Capnocytophaga and Leptotrichia, while C-MIP permanent dentition sites showed higher prevalence of Filifactor alocis. Aggregatibacter actinomycetemcomitans (Aa) was among species seen in high prevalence and levels in both primary and permanent C-MIP sites. Moreover, both permanent and primary C-MIP sites showed distinct microbial communities when compared to dentition-matched healthy sites in the same subject (p < 0.01).
CONCLUSIONS: Primary and permanent teeth with C-MIP showed a dysbiotic microbiome, with children affected in the primary dentition showing a distinct profile from those affected in the permanent dentition. However, Aa was enriched in both primary and permanent diseased sites, confirming that this microorganism is implicated in C-MIP in both dentitions.
摘要：
背景：本研究的目的是评估影响原发性或永久性牙列的C级磨牙切牙型牙周炎（C-MIP）患者的龈下微生物组。
方法：从45例C-MIP患者患病和健康部位的龈下生物膜样品中分离DNA，并进行系统发育微阵列分析。比较了原发性儿童与永久性牙列儿童之间的C-MIP位点。还评估了受C-MIP影响的部位和牙列匹配的健康部位之间的受试者内差异。
结果：受原牙列影响的受试者的C-MIP位点显示出与C-MIP恒牙列位点部分重叠但不同的微生物群落（p<0.05）。差异是由于Capnocytophaga和Leptotrichia属某些物种的主要C-MIP位点水平升高，而C-MIP永久牙列部位显示出较高的Filifactoralocis患病率。在主要和永久性C-MIP位点中，聚集杆菌放线菌（Aa）的流行率和水平都很高。此外，与同一受试者中与牙列匹配的健康部位相比，永久性和原发性C-MIP部位均显示出不同的微生物群落（p<0.01）。
结论：患有C-MIP的乳牙和恒牙显示出生态失调的微生物组，原牙列受影响的儿童与恒牙列受影响的儿童表现出不同的特征。然而,Aa在原发性和永久性患病部位都得到了丰富，证实该微生物在两个牙列中都与C-MIP有关。
方法：从45例C-MIP患者患病和健康部位的龈下生物膜样品中分离DNA，并进行系统发育微阵列分析。比较了原发性儿童与永久性牙列儿童之间的C-MIP位点。还评估了受C-MIP影响的部位和牙列匹配的健康部位之间的受试者内差异。
结果：受原牙列影响的受试者的C-MIP位点显示出与C-MIP恒牙列位点部分重叠但不同的微生物群落（p<0.05）。差异是由于Capnocytophaga和Leptotrichia属某些物种的主要C-MIP位点水平升高，而C-MIP永久牙列部位显示出较高的Filifactoralocis患病率。在主要和永久性C-MIP位点中，聚集杆菌放线菌（Aa）的流行率和水平都很高。此外，与同一受试者中与牙列匹配的健康部位相比，永久性和原发性C-MIP部位均显示出不同的微生物群落（p<0.01）。
结论：患有C-MIP的乳牙和恒牙显示出生态失调的微生物组，原牙列受影响的儿童与恒牙列受影响的儿童表现出不同的特征。然而,Aa在原发性和永久性患病部位都得到了丰富，证实该微生物在两个牙列中都与C-MIP有关。
