{Reference Type}: Journal Article
{Title}: Evaluation of microbiome in primary and permanent dentition in grade C periodontitis in young individuals.
{Author}: Koo SS;Fernandes JG;Li L;Huang H;Aukhil I;Harrison P;Diaz PI;Shaddox LM;
{Journal}: J Periodontol
{Volume}: 95
{Issue}: 7
{Year}: 2024 Jul 13
{Factor}: 4.494
{DOI}: 10.1002/JPER.23-0504
{Abstract}: <B>BACKGROUND: </B>The aim of the present study was to evaluate the subgingival microbiome in patients with grade C molar-incisor pattern periodontitis (C-MIP) affecting the primary or permanent dentitions.<BR><B>METHODS: </B>DNA was isolated from subgingival biofilm samples from diseased and healthy sites from 45 C-MIP patients and subjected to phylogenetic microarray analysis. C-MIP sites were compared between children affected in the primary to those affected in the permanent dentitions. Within-subject differences between C-MIP-affected sites and dentition-matched healthy sites were also evaluated.<BR><B>RESULTS: </B>C-MIP sites of subjects affected in the primary dentition showed partially overlapping but distinct microbial communities from C-MIP permanent dentition sites (p < 0.05). Differences were due to increased levels in primary C-MIP sites of certain species of the genera Capnocytophaga and Leptotrichia, while C-MIP permanent dentition sites showed higher prevalence of Filifactor alocis. Aggregatibacter actinomycetemcomitans (Aa) was among species seen in high prevalence and levels in both primary and permanent C-MIP sites. Moreover, both permanent and primary C-MIP sites showed distinct microbial communities when compared to dentition-matched healthy sites in the same subject (p < 0.01).<BR><B>CONCLUSIONS: </B>Primary and permanent teeth with C-MIP showed a dysbiotic microbiome, with children affected in the primary dentition showing a distinct profile from those affected in the permanent dentition. However, Aa was enriched in both primary and permanent diseased sites, confirming that this microorganism is implicated in C-MIP in both dentitions.