■唾液咖啡因(1,3,7-三甲基嘌呤-2,6-二酮或1,3,7-三甲基黄嘌呤)清除率的测量可以,原则上,用于评估肝功能,诊断慢性肝病,并对儿童肝细胞色素P450(CYP)同工酶的底物进行调查,无需静脉穿刺。然而,关于肝CYP亚型的儿童期性二态性知之甚少。此外,协会,如果有的话,唾液咖啡因清除率与儿童年龄之间的关系迄今尚未确定。这项研究的目的是评估男孩和女孩的唾液咖啡因清除率是否不同,以及儿童时期是否随年龄而变化。
■在至少24小时后,9名男孩(平均年龄(标准误差)9.6(1.1)y)和8名女孩(平均年龄11.0(1.2)y),他们都没有吸烟或患有慢性肝病,摄入了按体重滴定的口服咖啡因剂量,即3mgkg-1。2小时和14小时后收集的唾液样品进行分光光度咖啡因分析。
■男孩和女孩年龄匹配。男孩的平均咖啡因清除率为2.47(0.33)mLmin-1kg-1,而女孩的平均咖啡因清除率为2.20(0.31)mLmin-1kg-1(p=0.56)。唾液咖啡因清除率与年龄呈负相关(r=-0.59,p=0.01)。
■在考虑儿童唾液咖啡因清除率或对儿童进行CYP1A2和黄嘌呤氧化酶底物调查时,按性别分层似乎是不必要的。此外,儿童唾液咖啡因清除率与年龄呈负相关.
UNASSIGNED: Measurement of salivary caffeine (1,3,7-trimethylpurine-2,6-dione or 1,3,7-trimethylxanthine) clearance can, in principle, be used to assess hepatic function, diagnose chronic hepatic disease and conduct investigations of substrates of hepatic cytochrome P450 (CYP) isozymes in children, without recourse to venepuncture. However, little is known about childhood sexual dimorphism of hepatic CYP isoforms. Furthermore, the association, if any, between salivary caffeine clearance and age in children has not hitherto been established. The aims of this study were to assess whether salivary caffeine clearance differs between boys and girls and whether it varies with age during childhood.
UNASSIGNED: Following at least 24 h\' abstinence from dietary caffeine, nine boys (mean (standard error) age 9.6 (1.1) y) and eight girls (mean age 11.0 (1.2) y), none of whom was a smoker or suffered from chronic hepatic disease, ingested an oral caffeine dose titrated by body mass, namely 3 mg kg-1. Salivary samples collected two and 14 h later underwent spectrophotometric caffeine analysis.
UNASSIGNED: The boys and the girls were age matched. The mean caffeine clearance in the boys was 2.47 (0.33) mL min-1 kg-1, while that in the girls was 2.20 (0.31) mL min-1 kg-1 (p = 0.56). The salivary caffeine clearance was negatively correlated with age (r = -0.59, p = 0.01).
UNASSIGNED: Stratification by sex appears to be unnecessary when considering childhood salivary caffeine clearance or when conducting investigations in children of CYP1A2 and xanthine oxidase substrates. Furthermore, childhood salivary caffeine clearance is negatively correlated with age.