背景:结肠腺癌(COAD)是一种高度异质性的疾病,这是女性第二常见的癌症,男性第三常见。I型胶原α2(COL1A2)已被证明参与多种肿瘤的癌变;然而,COAD中COL1A2的表达和预后意义及其潜在机制尚不清楚.
方法:COL1A2的一般概况,其表达模式,并通过各种生物信息学工具系统地评估预后价值。COAD患者的COL1A2蛋白水平采用免疫组织化学分析进行验证。此外,进行富集分析以探索COAD中COL1A2的可能调控途径。
结果:COAD中COL1A2的mRNA和蛋白水平明显高于正常组织(P<0.05)。COAD中COL1A2的表达随着癌症分期和淋巴结转移状态而增加,COL1A2基因启动子甲基化水平可能与其mRNA表达呈负相关。生存分析表明,COL1A2是区分疾病特异性生存(DSS)状态的可靠预测因子,总生存期(OS),和无进展生存期(PFS),并可能作为COAD患者DSS和OS的可靠独立预后生物标志物(P<0.05)。富集分析表明,粘着斑是COL1A2最可能的调节途径。
结论:总的来说,COL1A2作为独立的预后生物标志物,可能是COAD的潜在治疗靶点。
BACKGROUND: Colon adenocarcinoma (COAD) is a highly heterogeneous disease, which is the second most common cancer in females and third in males. Collagen type I alpha 2 (
COL1A2) has been documented to be involved in the carcinogenesis of multiple tumors; however, the expression and prognostic significance of
COL1A2 and its underlying mechanism in COAD remains unclarified.
METHODS: The general profile of
COL1A2, its expression pattern, and prognostic value were systematically assessed through various bioinformatics tools. The protein level of
COL1A2 was verified in COAD patients using immunohistochemistry analysis. In addition, enrichment analyses were performed to explore the possible regulatory pathways of COL1A2 in COAD.
RESULTS: The mRNA and protein levels of COL1A2 were significantly increased in COAD than that in normal tissues (P < 0.05). The COL1A2 expression tended to increase along with cancer stages and nodal metastasis status in COAD, while the promoter methylation levels of COL1A2 might negatively related to its mRNA expression. Survival analysis showed that COL1A2 was a reliable predictor for distinguishing the status of disease-specific survival (DSS), overall survival (OS), and progression-free survival (PFS), and might serve as a robust independent prognostic biomarker for DSS and OS in COAD patients (P < 0.05). The enrichment analysis showed focal adhesion as the most possible regulatory pathway by
COL1A2.
CONCLUSIONS: Collectively, COL1A2 functioned as an independent prognostic biomarker and might be a potential therapeutic target in COAD.