背景:先前的研究表明淋巴细胞和细胞因子可以介导骨代谢。本研究探讨了淋巴细胞和细胞因子水平与骨代谢的临床关联和预测能力。
方法:本研究共纳入162例患者。I型前胶原(P1NP)的N端前肽水平,β-胶原降解产物(β-CTX),总T淋巴细胞,未成熟T淋巴细胞,抑制/细胞毒性T淋巴细胞,辅助/诱导T淋巴细胞,B淋巴细胞,自然杀伤(NK)细胞,干扰素-γ(IFN-γ),肿瘤坏死因子-α(TNF-α),IFN-α,白细胞介素-1β(IL-1β),评估IL-2、IL-4、IL-5、IL-6、IL-8、IL-10和IL12p70。检查了这些淋巴细胞亚群和细胞因子与骨代谢状态之间的关系,并评估了它们对骨代谢状态的预测能力。
结果:主成分分析(PCA)和相关分析结果因不同骨代谢状态下淋巴细胞亚群和细胞因子的差异而异。差异分析显示B淋巴细胞绝对计数差异有统计学意义(P<0.05)。IL-12p70水平(P<0.05),和IL-8(P<0.001)在不同的P1NP水平。总T淋巴细胞绝对计数差异有统计学意义(P<0.05)。B淋巴细胞(P<0.05),IL-6水平(P<0.05),B淋巴细胞百分比(P<0.01),不同β-CTX水平的NK细胞(P<0.05)。此外,受试者工作特征(ROC)曲线显示,B淋巴细胞的绝对计数和IL-12p70和IL-8的水平可用于评估骨形成状态,而T和B淋巴细胞的绝对计数,IL-6水平以及NK细胞和B淋巴细胞的百分比可用于评估骨吸收状态。
结论:骨代谢状态根据淋巴细胞亚群和细胞因子水平而改变。差异表达的淋巴细胞和细胞因子可用于区分骨代谢状态。
BACKGROUND: Previous research has shown that lymphocytes and cytokines can mediate bone metabolism. This study explored the clinical association and predictive ability of lymphocytes and cytokines levels for bone metabolism.
METHODS: A total of 162 patients were enrolled in this study. The levels of N-terminal propeptide of type I procollagen (P1NP), β-collagen degradation product (β-CTX), total T lymphocytes, immature T lymphocytes, suppressor/cytotoxic T lymphocytes, helper/inducer T lymphocytes, B lymphocytes, natural killer (NK) cells, Interferon-gamma (IFN-γ), tumour necrosis factor-alpha (TNF-α), IFN-α, interleukin-1 beta (IL-1β), IL-2, IL-4, IL-5, IL-6, IL-8, IL-10, and IL12p70 were evaluated. The relationship between these lymphocyte subsets and cytokines with bone metabolic status was examined and their predictive ability for bone metabolic status was assessed.
RESULTS: The principal component analysis (PCA) and correlation analysis results varied on differences in lymphocyte subsets and cytokines in various bone metabolism states. Differential analysis revealed significant differences in the absolute counts of B lymphocytes (P < 0.05), level of IL-12p70 (P < 0.05), and IL-8 (P < 0.001) at different P1NP levels. Significant differences were observed in the absolute counts of total T lymphocytes (P < 0.05), B lymphocytes (P < 0.05), the level of IL-6 (P < 0.05), the percentage of B lymphocytes (P < 0.01), and NK cells (P < 0.05) at different β-CTX levels. Furthermore, the receiver operating characteristic (ROC) curve showed that the absolute count of B lymphocytes and levels of IL-12p70 and IL-8 could be used to evaluate bone formation states, while the absolute counts of T and B lymphocytes, level of IL-6, and percentages of NK cells and B lymphocytes could be used to evaluate bone resorption states.
CONCLUSIONS: The bone metabolism status changed based on the lymphocyte subsets and cytokine levels. Differentially expressed lymphocytes and cytokines could be used to distinguish bone metabolism status.