PDF(Pubmed)
Abstract：
OBJECTIVE: This study aimed to investigate the effect of amorphous calcium carbonate (ACC) supplementation on bone growth in growing rats.
METHODS: We used 3-week-old male Wistar rats to simulate childhood and adolescent growth stages. Rats were divided into four groups as follows: a control group (C), a low-dose group (L, 20.65 mg/kg body weight (BW) ACC), a medium-dose group (M, 206.5 mg/kg BW ACC), and a high-dose group (H, 413 mg/kg BW ACC) administered by gavage. Body length (BL) and BW were measured weekly. The bone mineral density (BMD) of two lumbar vertebrae (L3 and L4) and the left femur were analyzed by micro-computed tomography (μCT) at 0, 4, 8, and 12 weeks. At the end of 12 weeks, the rats were sacrificed. After that, blood samples were collected from the abdominal aorta. Femurs and tibias were collected and weighed, and their lengths were measured. Then, bone samples were used to perform histopathological and histomorphometric analyses.
RESULTS: It showed that ACC supplementation in growing rats increased the trabecular bone thickness and serum bone formation biomarkers. Furthermore, high-dose ACC decreased serum bone resorption biomarkers and increased BMD.
CONCLUSIONS: ACC supplementation can enhance osteoblast metabolism and inhibit osteoclast metabolism, resulting in a higher bone formation rate compared to bone resorption. This led to increased trabecular bone thickness, a higher BMD, and supported bone growth.
METHODS: We used 3-week-old male Wistar rats to simulate childhood and adolescent growth stages. Rats were divided into four groups as follows: a control group (C), a low-dose group (L, 20.65 mg/kg body weight (BW) ACC), a medium-dose group (M, 206.5 mg/kg BW ACC), and a high-dose group (H, 413 mg/kg BW ACC) administered by gavage. Body length (BL) and BW were measured weekly. The bone mineral density (BMD) of two lumbar vertebrae (L3 and L4) and the left femur were analyzed by micro-computed tomography (μCT) at 0, 4, 8, and 12 weeks. At the end of 12 weeks, the rats were sacrificed. After that, blood samples were collected from the abdominal aorta. Femurs and tibias were collected and weighed, and their lengths were measured. Then, bone samples were used to perform histopathological and histomorphometric analyses.
RESULTS: It showed that ACC supplementation in growing rats increased the trabecular bone thickness and serum bone formation biomarkers. Furthermore, high-dose ACC decreased serum bone resorption biomarkers and increased BMD.
CONCLUSIONS: ACC supplementation can enhance osteoblast metabolism and inhibit osteoclast metabolism, resulting in a higher bone formation rate compared to bone resorption. This led to increased trabecular bone thickness, a higher BMD, and supported bone growth.
摘要：
目的：本研究旨在研究补充无定形碳酸钙（ACC）对生长大鼠骨骼生长的影响。
方法：我们使用3周龄雄性Wistar大鼠来模拟童年和青春期的生长阶段。将大鼠分为四组：对照组（C），低剂量组（L，20.65mg/kg体重（BW）ACC），中等剂量组(M，206.5mg/kgBWACC)，和高剂量组（H，413mg/kgBWACC）通过管饲法给药。每周测量体长(BL)和BW。在0、4、8和12周时，通过显微计算机断层扫描（μCT）分析了两个腰椎（L3和L4）和左股骨的骨矿物质密度（BMD）。在12周结束时，大鼠被处死。之后,从腹主动脉采集血样.收集股骨和胫骨并称重，并测量了它们的长度。然后，骨样本用于进行组织病理学和组织形态学分析.
结果：它表明在生长的大鼠中补充ACC增加了小梁骨厚度和血清骨形成生物标志物。此外，大剂量ACC降低血清骨吸收生物标志物和增加BMD。
结论：补充ACC能增强成骨细胞代谢，抑制破骨细胞代谢，导致与骨吸收相比更高的骨形成速率。这导致骨小梁厚度增加，更高的BMD,并支持骨骼生长。
方法：我们使用3周龄雄性Wistar大鼠来模拟童年和青春期的生长阶段。将大鼠分为四组：对照组（C），低剂量组（L，20.65mg/kg体重（BW）ACC），中等剂量组(M，206.5mg/kgBWACC)，和高剂量组（H，413mg/kgBWACC）通过管饲法给药。每周测量体长(BL)和BW。在0、4、8和12周时，通过显微计算机断层扫描（μCT）分析了两个腰椎（L3和L4）和左股骨的骨矿物质密度（BMD）。在12周结束时，大鼠被处死。之后,从腹主动脉采集血样.收集股骨和胫骨并称重，并测量了它们的长度。然后，骨样本用于进行组织病理学和组织形态学分析.
结果：它表明在生长的大鼠中补充ACC增加了小梁骨厚度和血清骨形成生物标志物。此外，大剂量ACC降低血清骨吸收生物标志物和增加BMD。
结论：补充ACC能增强成骨细胞代谢，抑制破骨细胞代谢，导致与骨吸收相比更高的骨形成速率。这导致骨小梁厚度增加，更高的BMD,并支持骨骼生长。
