聚合物在医疗领域的广泛使用促进了各种设备和植入物的发展,有助于器官功能的恢复。然而,尽管它们具有生物相容性和鲁棒性等优点,这些材料经常面临细菌污染和随后的炎症等挑战,导致植入物相关感染(IAI)。有效整合植入物对于防止细菌定植和减少炎症反应至关重要。为了克服这些重大问题,表面化学修饰已被广泛探索。的确,点击化学,尤其是,铜(I)催化的叠氮化物-炔环加成(CuAAC)反应已成为一种有前途的表面官能化方法,而不会影响材料的整体性能。姜黄素,以其多样化的生物活动而闻名,低溶解度和稳定性。为了提高其生物利用度,生物共轭策略近年来引起了人们的关注。这项研究代表了将姜黄素衍生物固定到聚对苯二甲酸乙二醇酯(PET)表面上的开创性工作,旨在对抗细菌粘附,炎症和凝血。在姜黄素衍生物生物缀合之前,荧光团,丹磺酰衍生物,用于监测和确定拟议方法的效率。需要先前的表面化学修饰来固定丹磺酰和姜黄素衍生物。紫外可见(UV-Vis)证明了PET表面的酰胺化官能化。其他表面表征技术,包括X射线光电子能谱(XPS),衰减全反射傅里叶变换红外(ATR-FTIR),扫描电子显微镜(SEM)和接触角,其中,还证实了丹磺酰和姜黄素衍生物的缀合。另一方面,不同的生物学试验证实姜黄素衍生物固定的PET表面不表现出细胞毒性作用。此外,进行相应的炎症测试,表明这些聚合物表面不会产生炎症,当姜黄素衍生物被固定时,它们降低炎症标志物水平(IL-6)。此外,测量革兰氏阳性和革兰氏阴性细菌的细菌生长,证明姜黄素衍生物在PET上的固定为材料提供了抗菌性能。最后,溶血率分析和全血凝血试验证明了PET-Cur表面的抗血栓形成作用,并且在制造的功能表面中没有溶血问题。
The extensive use of polymers in the medical field has facilitated the development of various devices and implants, contributing to the restoration of organ function. However, despite their advantages such as biocompatibility and robustness, these materials often face challenges like bacterial contamination and subsequent inflammation, leading to implant-associated infections (IAI). Integrating implants effectively is crucial to prevent bacterial colonization and reduce inflammatory responses. To overcome these major issues, surface chemical modifications have been extensively explored. Indeed, click chemistry, and particularly, copper (I)-catalyzed azide-alkyne cycloaddition (CuAAC) reaction has emerged as a promising approach for surface functionalization without affecting material bulk properties. Curcumin, known for its diverse biological activities, suffers from low solubility and stability. To enhance its bioavailability,
bioconjugation strategy has garnered attention in recent years. This study represents pioneering work in immobilizing curcumin derivative onto polyethylene terephthalate (PET) surfaces, aiming to combat bacterial adhesion, inflammation and coagulation. Before curcumin derivative
bioconjugation, a fluorophore, dansyl derivative, was employed in order to monitor and determine the efficiency of the proposed methodology. Previous surface chemical modifications were required for the immobilization of both dansyl and curcumin derivatives. Ultraviolet-Visible (UV-Vis) demonstrated the amidation functionalization of PET surface. Other surface characterization techniques including X-ray Photoelectron Spectroscopy (XPS), Attenuated Total Reflectance Fourier Transformed Infrared (ATR-FTIR), Scanning Electron Microscopy (SEM) and contact angle, among others, confirmed also the conjugation of both dansyl and curcumin derivatives. On the other hand, different biological assays corroborated that curcumin derivative immobilized PET surfaces do not exhibit cytotoxicity effect. Additionally, corresponding inflammation test were performed, indicating that these polymeric surfaces do not produce inflammation and, when curcumin derivative is immobilized, they decrease the inflammation marker level (IL-6). Moreover, the bacterial growth of both Gram positive and Gram negative bacteria were measured, demonstrating that the immobilization of curcumin derivative on PET provided antibacterial properties to the material. Finally, hemolysis rate analysis and whole blood clotting assay demonstrated the antithrombogenic effect of PET-Cur surfaces as well as no hemolysis concern in the fabricated functional surfaces.