在研究中,颗粒形式的超顺磁性荧光生物活性玻璃的制造,纳米纤维,3D支架是通过包括磁赤铁矿(γ-Fe2O3)纳米颗粒和光致发光稀土元素离子(Eu3,Gd3+,和Yb3+)使用溶胶-凝胶,静电纺丝,和机器人铸造技术,分别。磁性荧光生物活性玻璃对骨肉瘤SaOS-2,前成骨细胞MC3T3-E1和BJ成纤维细胞的体外细胞毒性,以及它们的溶血活性和甲苯磺酸索拉非尼的负载和释放行为,被调查了。使用MTT测定法测试生物活性玻璃样品的细胞毒性。此外,检查了所研究眼镜的碱性磷酸酶活性随时间的变化。使用茜素红S染色分析了成骨细胞接种前的玻璃样品的矿化行为。结果表明,以颗粒和纳米纤维形式研究的生物活性玻璃的体外细胞毒性取决于样品浓度,而在3D支架的情况下,在骨肉瘤上没有观察到细胞毒性反应,前成骨细胞,和成纤维细胞。同样,基于颗粒和纳米纤维的玻璃样品诱导红细胞的剂量依赖性溶血。与基于颗粒和纳米纤维的样品相比,3D支架的载药率低得多。药物释放率从25%到90%不等,取决于生物活性玻璃形态和释放介质的pH。结论是,所研究的生物活性玻璃具有用于组织工程应用和癌症治疗的潜力。
In the study, the fabrication of superparamagnetic-fluorescent bioactive glasses in the form of the particle, nanofiber, and 3D scaffolds was performed by including maghemite (γ-Fe2O3) nanoparticles and photoluminescent rare earth element ions (Eu3+, Gd3+, and Yb3+) using sol-gel, electrospinning, and robocasting techniques, respectively. The in vitro cytotoxicity of the magnetic-fluorescent bioactive glasses on osteosarcoma SaOS-2, pre-osteoblast MC3T3-E1, and BJ fibroblast cells, as well as their hemolytic activity and sorafenib tosylate loading and release behavior, were investigated. The cytotoxicity of the bioactive glass samples was tested using the MTT assay. Additionally, the alkaline phosphatase activity of the studied glasses was examined as a function of time. The mineralization behavior of the pre-osteoblast cell-seeded glass samples was analyzed using Alizarin red S staining. Results revealed that the in vitro cytotoxicity of the studied bioactive glasses in the form of particles and nanofibers depended on the sample concentration, whereas in the case of the 3D scaffolds, no cytotoxic response was observed on the osteosarcoma, pre-osteoblast, and fibroblast cells. Similarly, particle and nanofiber-based glass samples induced dose-dependent hemolysis on red blood cells. Drug loading rates were much lower for the 3D scaffolds compared to the particle and nanofiber-based samples. Drug release rates ranged from 25 % to 90 %, depending on the bioactive glass morphology and the pH of the release medium. It was concluded that the studied bioactive glasses have the potential to be used in tissue engineering applications and cancer therapy.