Abstract：
The applications of ferrimagnetic nanoparticles (F-MNPs) in magnetic hyperthermia (MH) are restricted by their stabilization in microscale aggregates due to magnetostatic interactions significantly reducing their heating performances. Coating the F-MNPs in a silica layer is expected to significantly reduce the magnetostatic interactions, thereby increasing their heating ability. A new fast, facile, and eco-friendly oil-in-water microemulsion-based method was used for coating Zn0.4Fe2.6O4 F-MNPs in a silica layer within 30 min by using ultrasounds. The silica-coated clusters were characterized by various physicochemical techniques and MH, while cytotoxicity studies, cellular uptake determination, and in vitro MH experiments were performed on normal and malignant cell lines. The average hydrodynamic diameter of silica-coated clusters was approximately 145 nm, displaying a high heating performance (up to 2600 W/gFe). Biocompatibility up to 250 μg/cm2 (0.8 mg/mL) was recorded by Alamar Blue and Neutral Red assays. The silica-coating increases the cellular uptake of Zn0.4Fe2.6O4 clusters up to three times and significantly improves their intracellular MH performances. A 90% drop in cellular viability was recorded after 30 min of MH treatment (20 kA/m, 355 kHz) for a dosage level of 62.5 μg/cm2 (0.2 mg/mL), while normal cells were more resilient to MH treatment.
摘要：
铁磁纳米粒子（F-MNPs）在磁热（MH）中的应用受到其在微尺度聚集体中的稳定性的限制，这是由于静磁相互作用显着降低了其加热性能。将F-MNPs涂覆在二氧化硅层中预计会显著减少静磁相互作用，从而提高其加热能力。一个新的快，轻而易举，并使用环保的水包油微乳液方法在30分钟内通过超声波将Zn0.4Fe2.6O4F-MNPs涂覆在二氧化硅层中。通过各种物理化学技术和MH，而细胞毒性研究，细胞摄取测定，并在正常和恶性细胞系上进行了体外MH实验。二氧化硅涂层团簇的平均流体动力学直径约为145nm，显示高加热性能（高达2600W/gFe）。通过AlamarBlue和中性红测定记录高达250μg/cm2(0.8mg/mL)的生物相容性。二氧化硅涂层可将Zn0.4Fe2.6O4簇的细胞摄取增加三倍，并显着改善其细胞内MH性能。MH处理30分钟后，细胞活力下降了90％（20kA/m，355kHz），剂量水平为62.5μg/cm2（0.2mg/mL），而正常细胞对MH治疗更有弹性。
