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Abstract：
UNASSIGNED: Kigelia africana (Lam.) Benth. (Bignoniaceae) syn. Kigelia pinnata (Jacq. DC) is a tropical plant that is native to tropical Africa. The aim of this study was to determine if a methanolic extract prepared from Kigelia africana (KAE) can promote wound healing in treated human normal epidermal keratinocyte (HaCaT) cells and human normal foreskin fibroblast cell line (BJ) cells compared with untreated cells.
UNASSIGNED: Experimental steps included: the methanolic extraction of the leaf and fruit of the Kigelia africana plant; the preparation of HaCaT and BJ cell lines; cell culture with a stable tetrazolium salt-based proliferation assay; and the evaluation of the wound healing effect of KAE (2μg/ml) in BJ and HaCaT cells. The phytochemical contents of KAE were determined using liquid chromatography quadrupole time-of-flight mass spectrometry.
UNASSIGNED: The following molecules were identified as being present in the KAE, among others: cholesterol sulfate; lignoceric acid; embelin; isostearic acid; linoleic acid; dioctyl phthalate; arg-pro-thr; 15-methyl-15(S)-PGE1; sucrose; benzododecinium (Ajatin); and 9-Octadecenamide (oleamide). KAE effected faster wound healing in treated cells compared with untreated cells for both cell lines. HaCaT cells that had been mechanically injured and treated with KAE healed completely in 48 hours compared with 72 hours for untreated HaCaT cells. Treated BJ cells healed completely in 72 hours compared with 96 hours for untreated BJ cells. Concentrations of KAE up to 300μg/ml had a very low cytotoxic effect on treated BJ and HaCaT cells.
UNASSIGNED: The experimental data in this study support the potential of KAE-based wound healing treatment to accelerate wound healing.
UNASSIGNED: Experimental steps included: the methanolic extraction of the leaf and fruit of the Kigelia africana plant; the preparation of HaCaT and BJ cell lines; cell culture with a stable tetrazolium salt-based proliferation assay; and the evaluation of the wound healing effect of KAE (2μg/ml) in BJ and HaCaT cells. The phytochemical contents of KAE were determined using liquid chromatography quadrupole time-of-flight mass spectrometry.
UNASSIGNED: The following molecules were identified as being present in the KAE, among others: cholesterol sulfate; lignoceric acid; embelin; isostearic acid; linoleic acid; dioctyl phthalate; arg-pro-thr; 15-methyl-15(S)-PGE1; sucrose; benzododecinium (Ajatin); and 9-Octadecenamide (oleamide). KAE effected faster wound healing in treated cells compared with untreated cells for both cell lines. HaCaT cells that had been mechanically injured and treated with KAE healed completely in 48 hours compared with 72 hours for untreated HaCaT cells. Treated BJ cells healed completely in 72 hours compared with 96 hours for untreated BJ cells. Concentrations of KAE up to 300μg/ml had a very low cytotoxic effect on treated BJ and HaCaT cells.
UNASSIGNED: The experimental data in this study support the potential of KAE-based wound healing treatment to accelerate wound healing.
摘要：
■非洲基格利亚（林。)第十。（比目鱼科）同系物。Kigeliapinnata（Jacq。DC）是一种热带植物，原产于热带非洲。这项研究的目的是确定与未处理的细胞相比，从非洲金鸡（KAE）制备的甲醇提取物是否可以促进治疗的人正常表皮角质形成细胞（HaCaT）细胞和人正常包皮成纤维细胞（BJ）细胞的伤口愈合。
■实验步骤包括:非洲基利亚植物的叶和果实的甲醇提取;HaCaT和BJ细胞系的制备;具有稳定的基于四唑盐的增殖测定的细胞培养;以及KAE(2μg/ml)在BJ和HaCaT细胞中的伤口愈合效果的评估。使用液相色谱四极杆飞行时间质谱法测定KAE的植物化学物质含量。
■以下分子被鉴定为存在于KAE中，除其他外：胆固醇硫酸盐；二十四酸；embelin；异硬脂酸；亚油酸；邻苯二甲酸二辛酯；arg-pro-thr；15-甲基-15（S）-PGE1；蔗糖；苯并十二铵（Ajatin）；和9-十八碳酰胺（油酰胺）。对于两种细胞系,与未处理的细胞相比,KAE在处理的细胞中实现更快的伤口愈合。与未处理的HaCaT细胞72小时相比,机械损伤并用KAE处理的HaCaT细胞在48小时内完全愈合。与未处理的BJ细胞的96小时相比,处理的BJ细胞在72小时内完全愈合。高达300μg/ml的KAE浓度对经处理的BJ和HaCaT细胞具有非常低的细胞毒性作用。
■本研究中的实验数据支持基于KAE的伤口愈合治疗加速伤口愈合的潜力。
■实验步骤包括:非洲基利亚植物的叶和果实的甲醇提取;HaCaT和BJ细胞系的制备;具有稳定的基于四唑盐的增殖测定的细胞培养;以及KAE(2μg/ml)在BJ和HaCaT细胞中的伤口愈合效果的评估。使用液相色谱四极杆飞行时间质谱法测定KAE的植物化学物质含量。
■以下分子被鉴定为存在于KAE中，除其他外：胆固醇硫酸盐；二十四酸；embelin；异硬脂酸；亚油酸；邻苯二甲酸二辛酯；arg-pro-thr；15-甲基-15（S）-PGE1；蔗糖；苯并十二铵（Ajatin）；和9-十八碳酰胺（油酰胺）。对于两种细胞系,与未处理的细胞相比,KAE在处理的细胞中实现更快的伤口愈合。与未处理的HaCaT细胞72小时相比,机械损伤并用KAE处理的HaCaT细胞在48小时内完全愈合。与未处理的BJ细胞的96小时相比,处理的BJ细胞在72小时内完全愈合。高达300μg/ml的KAE浓度对经处理的BJ和HaCaT细胞具有非常低的细胞毒性作用。
■本研究中的实验数据支持基于KAE的伤口愈合治疗加速伤口愈合的潜力。
