未经授权:使用经巩膜光学成像(TOPI)对体内健康的视网膜色素上皮(RPE)细胞进行成像,并分析RPE细胞特征随年龄变化的统计数据,轴向长度(AL),和偏心。
UNASSIGNED:单中心,探索性,prospective,和描述性临床研究。
UNASISIGNED:29只眼睛(AL:24.03±0.93毫米;范围:21.9-26.7毫米),年龄在21至70岁(37.1±13.3岁;19名男性,10名妇女)。
未经证实:视网膜图像,包括眼底摄影和谱域OCT,AL,和屈光不正测量在基线收集。对于每一只眼睛,使用TOPI在不同位置获取6张高分辨率RPE图像,其中一个被成像5次,以评估该方法的可重复性。在TOPI后1至3周重复随访眼科检查以评估安全性。用定制的自动化软件分析视网膜色素上皮图像以提取细胞参数。所选择的高对比度图像的统计分析包括计算每次重复时每个特征的变异系数(CoV)以及Spearman和Mann-Whitney测试以研究细胞特征与眼睛和受试者特征之间的关系。
未经证实:视网膜色素上皮细胞特征:密度,area,中心到中心的间距,邻居的数量,循环性,伸长率,坚固性,和边界距离CoV。
UNASSIGNED:从TOPI图像中以距中央凹1.6°至16.3°的偏心率提取黄斑RPE细胞特征。对于每个功能,平均CoV<4%。Spearman检验显示RPE细胞特征内的相关性。在前凹中,为所有参与者选择图像的区域,较长的AL与RPE细胞密度降低显著相关(RSpearman,Rs=-0.746;P<0.0001)和细胞面积增加(Rs=0.668;P<0.0001),没有形态学变化。衰老还与RPE密度降低(Rs=-0.391;P=0.036)和细胞面积增加(Rs=0.454;P=0.013)显着相关。下圆形,不太对称,更细长,在那些>50年中观察到较大的细胞。
UNASSIGNED:TOPI技术对CoV的体内RPE细胞成像的重复性<4%,并用于分析生理因素对健康志愿者周围凹RPE细胞形态的影响。
UNASSIGNED:在参考文献之后可以找到专有或商业披露。
UNASSIGNED: To image healthy retinal pigment epithelial (RPE) cells in vivo using Transscleral OPtical Imaging (TOPI) and to analyze statistics of RPE cell features as a function of age, axial length (AL), and eccentricity.
UNASSIGNED: Single-center, exploratory, prospective, and descriptive clinical study.
UNASSIGNED: Forty-nine eyes (AL: 24.03 ± 0.93 mm; range: 21.9-26.7 mm) from 29 participants aged 21 to 70 years (37.1 ± 13.3 years; 19 men, 10 women).
UNASSIGNED: Retinal images, including fundus photography and spectral-domain OCT, AL, and refractive error measurements were collected at baseline. For each eye, 6 high-resolution RPE images were acquired using TOPI at different locations, one of them being imaged 5 times to evaluate the repeatability of the method. Follow-up ophthalmic examination was repeated 1 to 3 weeks after TOPI to assess safety. Retinal pigment epithelial images were analyzed with a custom automated software to extract cell parameters. Statistical analysis of the selected high-contrast images included calculation of coefficient of variation (CoV) for each feature at each repetition and Spearman and Mann-Whitney tests to investigate the relationship between cell features and eye and subject characteristics.
UNASSIGNED: Retinal pigment epithelial cell features: density, area, center-to-center spacing, number of neighbors, circularity, elongation, solidity, and border distance CoV.
UNASSIGNED: Macular RPE cell features were extracted from TOPI images at an eccentricity of 1.6° to 16.3° from the fovea. For each feature, the mean CoV was < 4%. Spearman test showed correlation within RPE cell features. In the perifovea, the region in which images were selected for all participants, longer AL significantly correlated with decreased RPE cell density (R Spearman, Rs = -0.746; P < 0.0001) and increased cell area (Rs = 0.668; P < 0.0001), without morphologic changes. Aging was also significantly correlated with decreased RPE density (Rs = -0.391; P = 0.036) and increased cell area (Rs = 0.454; P = 0.013). Lower circular, less symmetric, more elongated, and larger cells were observed in those > 50 years.
UNASSIGNED: The TOPI technology imaged RPE cells in vivo with a repeatability of < 4% for the CoV and was used to analyze the influence of physiologic factors on RPE cell morphometry in the perifovea of healthy volunteers.
UNASSIGNED: Proprietary or commercial disclosure may be found after the references.