单萜氧化物,桉树脑(1,8-桉树脑),桉树油的主要成分,已经在药理学上评估了抗炎和镇痛活性。目前的研究旨在评估桉树醇在完全弗氏佐剂诱导的类似人类类风湿性关节炎的关节炎中的抗关节炎潜力。大鼠左后足垫注射0.1mLCFA后出现多关节炎。口服各种剂量(100、200和400mg/kg)的桉树脑显著减少了爪水肿,身体体重减轻,5-LOX,PGE2和抗CCP水平。实时荧光定量PCR检测显示COX-2、TNF-α、NF-κB,在桉树脑处理组中IL-17、IL-6、IL-1β和IL-4和IL-10的上调。桉树脑治疗后血红蛋白和红细胞计数显着增加,而ESR,CRP,WBCs和血小板计数显著下降。桉树脑显着增加超氧化物歧化酶,然而,与CFA诱导的关节炎对照相比,过氧化氢酶和谷胱甘肽水平处理后MDA显著降低。Further,施用桉树脑的啮齿动物踝关节的影像学和组织病理学检查显示关节结构有所改善。吡罗昔康作为标准。此外,分子对接研究结果支持桉树脑抗关节炎功效表现出与IL-17,TNF-α,IL-4,IL-10,iNOS,NF-κB,5-LOX,和COX-2。桉树醇通过促进抗炎细胞因子如IL-4、IL-10和通过抑制促炎细胞因子如5-LOX,降低了CFA诱导的关节炎的严重程度。COX-2,IL-17,NF-κB,TNF-α,IL-6和IL-1β。因此,桉树脑因其显著的抗氧化和抗关节炎活性而可能作为潜在的治疗剂。
The monoterpene oxide, Eucalyptol (1,8-Cineole), a primary component of eucalyptus oil, has been evaluated pharmacologically for anti-inflammatory and analgesic activity. Current research aimed to evaluate Eucalyptol\'s anti-arthritic potential in a Complete Freund\'s adjuvant induced arthritis that resembles human rheumatoid arthritis. Polyarthritis developed after 0.1 mL CFA injection into the left hind footpad in rats. Oral administration of Eucalyptol at various doses (100, 200 and 400 mg/kg) significantly reduced paw edema, body weight loss, 5-LOX, PGE2 and Anti-CCP levels. Real-time PCR investigation showed significant downregulation of COX-2, TNF-α, NF-κB, IL-17, IL-6, IL-1β and upregulation of IL-4 and IL-10 in Eucalyptol treated groups. Hemoglobin and RBCs counts significantly increased post-treatment with Eucalyptol while ESR, CRP, WBCs and platelets count significantly decreased. Eucalyptol significantly increased Superoxide Dismutase, Catalase and Glutathione levels compared to CFA-induced arthritic control however, MDA significantly decreased post-treatment. Further, radiographic and histopathological examination of the ankle joints of rodents administered Eucalyptol revealed an improvement in the structure of the joints. Piroxicam was taken as standard. Furthermore, molecular docking findings supported the anti-arthritic efficacy of Eucalyptol exhibited high binding interaction against IL-17, TNF-α, IL-4, IL-10, iNOS NF-κB, 5-LOX, and COX-2. Eucalyptol has reduced the severity of CFA induced arthritis by promoting anti-inflammatory cytokines for example IL-4, IL-10 and by inhibiting pro-inflammatory cytokines such as 5-LOX, COX-2, IL-17, NF-κB, TNF-α, IL-6 and IL-1β. Therefore, Eucalyptol might be as a potential therapeutic agent because of its pronounced anti-oxidant and anti-arthritic activity.