PDF(Pubmed)
Abstract：
Lipid metabolism is an important part of the heart\'s energy supply. The expression pattern and molecular mechanism of lipid metabolism-related genes (LMRGs) in acute myocardial infarction (AMI) are still unclear, and the link between lipid metabolism and immunity is far from being elucidated. In this study, 23 Common differentially expressed LMRGs were discovered in the AMI-related mRNA microarray datasets GSE61144 and GSE60993. These genes were mainly related to \"leukotriene production involved in inflammatory response\", \"lipoxygenase pathway\", \"metabolic pathways\", and \"regulation of lipolysis in adipocytes\" pathways. 12 LMRGs (ACSL1, ADCY4, ALOX5, ALOX5AP, CCL5, CEBPB, CEBPD, CREB5, GAB2, PISD, RARRES3, and ZNF467) were significantly differentially expressed in the validation dataset GSE62646 with their AUC > 0.7 except for ALOX5AP (AUC = 0.699). Immune infiltration analysis and Pearson correlation analysis explored the immune characteristics of AMI, as well as the relationship between these identified LMRGs and immune response. Lastly, the up-regulation of ACSL1, ALOX5AP, CEBPB, and GAB2 was confirmed in the mouse AMI model. Taken together, LMRGs ACSL1, ALOX5AP, CEBPB, and GAB2 are significantly upregulated in AMI patients\' blood, peripheral blood of AMI mice, myocardial tissue of AMI mice, and therefore might be new potential biomarkers for AMI.
摘要：
脂质代谢是心脏能量供应的重要组成部分。脂质代谢相关基因(LMRGs)在急性心肌梗死(AMI)中的表达模式和分子机制尚不清楚。脂质代谢和免疫之间的联系还远未阐明。在这项研究中,23在AMI相关的mRNA微阵列数据集GSE61144和GSE60993中发现了常见的差异表达的LMRG。这些基因主要与“参与炎症反应的白三烯产生”有关“脂氧合酶途径”，“代谢途径”，和“脂肪细胞脂解调节”途径。12个LMRGs(ACSL1,ADCY4,ALOX5,ALOX5AP,CCL5,CEBPB,CEBPD,CREB5,GAB2,PISD,RARRES3和ZNF467）在验证数据集GSE62646中显著差异表达，其AUC>0.7，ALOX5AP除外（AUC=0.699）。免疫浸润分析和Pearson相关分析探讨AMI的免疫特性,以及这些鉴定的LMRGs与免疫应答之间的关系。最后,ACSL1、ALOX5AP的上调，CEBPB,在小鼠AMI模型中证实了GAB2。一起来看,LMRGsACSL1,ALOX5AP,CEBPB,和GAB2在AMI患者血液中显著上调，AMI小鼠外周血,AMI小鼠的心肌组织，因此可能是AMI新的潜在生物标志物。
