背景:在附睾,上皮细胞管理管腔环境的变化,以实现适当的精子成熟。此外,aquaglyceroporoins,水通道蛋白(AQP)的一个亚组,调节水的运输,甘油和上皮细胞中的其他小分子。
目的:我们的目的是表征衬里上皮,量化其细胞组成,并与三种野生反刍动物的附睾导管一起对AQP3,AQP7,AQP9和AQP10进行免疫定位,并确定物种特异性差异是否与尾管精子冷冻抗性变化有关。
方法:来自伊比利亚ibex的附睾(n=5),获得了mouflon(n=5)和chamois(n=6)。收集cauda精子,并在冷冻前后分析精子参数。在caput中进行AQP3,7,9,10和T-CD3的组织学和免疫组织化学,体和尾附睾区。
结果:这项工作首先描述了伊比利亚地区的衬里上皮,沿着三个解剖区域的mouflon和chomais附睾,由校长组成,基底,顶端,透明和光环细胞。然而,与mouflon和chomois相比,ibex中每种细胞类型的百分比不同。所有晕细胞的阳性T-CD3免疫标记证实了它们的T淋巴细胞性质。Aquaglyceroporin表达模式在物种之间相似,除了AQP7和AQP10免疫定位的差异。确认了附睾精子冷冻抗性的物种特异性差异。
结论:三种野生反刍动物的附睾上皮在细胞类型和AQP免疫定位的相对数量上不同,最终似乎会影响尾管表皮精子的冷冻抗性。
结论:我们的研究提供了附睾衬里上皮中定量组成和AQP模式表达与精子冷冻抗性的相关性的信息。
BACKGROUND: In the epididymis, epithelial cells manage changes in the luminal environment for proper sperm maturation. Moreover,
aquaglyceroporins, a subgroup of aquaporins (AQP), modulate the transport of water, glycerol and other small molecules in epithelial cells.
OBJECTIVE: We aim to characterise the lining epithelium, quantify its cell composition and immunolocalise the
aquaglyceroporins AQP3, AQP7, AQP9 and AQP10 alongside the epididymal ductus of three wild ruminant species, and to determine if species-specific differences could be associated with cauda sperm cryoresistance variations.
METHODS: Epididymides from Iberian ibex (n =5), mouflon (n =5) and chamois (n =6) were obtained. Cauda spermatozoa were collected and sperm parameters were analysed before and after freezing. Histology and immunohistochemistry of AQP3, 7, 9, 10 and T-CD3 were performed in the caput, corpus and cauda epididymal regions.
RESULTS: This work first describes the lining epithelium in Iberian ibex, mouflon and chamois epididymis along the three anatomical regions, consisting of principal, basal, apical, clear and halo cells. However, the percentage of each cell type differed in ibex compared to mouflon and chamois. The positive T-CD3 immunolabeling of all the halo cells confirmed their T-lymphocyte nature. Aquaglyceroporin expression patterns were similar among species, except for differences in AQP7 and AQP10 immunolocalisation in ibex. Species-specific differences in epididymal sperm cryoresistance were confirmed.
CONCLUSIONS: The epididymal epithelium of the three wild ruminants differ in their relative number of cell types and AQP immunolocalisation, which ultimately appears to affect cauda epidydimal spermatozoa cryoresistance.
CONCLUSIONS: Our study provides information on the relevance of the quantitative composition and AQP pattern expression in epididymal lining epithelium on sperm cryoresistance.