PDF(Pubmed)
Abstract：
The increasing incidence of male infertility in humans and animals creates the need to search for new factors that significantly affect the course of reproductive processes. Therefore, the aim of this study was to determine the temporospatial expression of aquaglyceroporins (AQP3, AQP7 and AQP9) in the bovine (Bos taurus) reproductive system using immunohistochemistry and Western blotting. The study also included morphological analysis and identification of GATA-4. In brief, in immature individuals, AQP3 and AQP7 were found in gonocytes. In reproductive bulls, AQP3 was observed in spermatocytes and spermatogonia, while AQP7 was visible in all germ cells and the Sertoli cells. AQP7 and AQP9 were detected in the Leydig cells. Along the entire epididymis of reproductive bulls, aquaglyceroporins were visible, among others, in basal cells (AQP3 and AQP7), in epididymal sperm (AQP7) and in the stereocilia of the principal cells (AQP9). In males of all ages, aquaglyceroporins were identified in the principal and basal cells of the vas deferens. An increase in the expression of AQP3 in the testis and cauda epididymis and a decrease in the abundance of AQP7 in the vas deferens with age were found. In conclusion, age-related changes in the expression and/or distribution patterns of AQP3, AQP7 and AQP9 indicate the involvement of these proteins in the normal development and course of male reproductive processes in cattle.
摘要：
人类和动物中男性不育症发病率的增加导致需要寻找显着影响生殖过程的新因素。因此,这项研究的目的是使用免疫组织化学和蛋白质印迹法确定牛（Bostaurus）生殖系统中水甘油孔素（AQP3，AQP7和AQP9）的时空表达。该研究还包括GATA-4的形态学分析和鉴定。简而言之,在不成熟的个体中，在性腺细胞中发现AQP3和AQP7。在生殖公牛中，在精母细胞和精原细胞中观察到AQP3，而AQP7在所有生殖细胞和支持细胞中均可见。在睾丸间质细胞中检测到AQP7和AQP9。沿着繁殖公牛的整个附睾，aquaglyceroporoins是可见的，其中,在基底细胞（AQP3和AQP7）中，附睾精子（AQP7）和主要细胞的立体纤毛（AQP9）。在所有年龄段的男性中，在输精管的主要细胞和基底细胞中鉴定出了水细胞。发现随着年龄的增长，睾丸和附睾尾中AQP3的表达增加，输精管中AQP7的丰度降低。总之,AQP3，AQP7和AQP9的表达和/或分布模式的年龄相关变化表明这些蛋白质参与了牛的正常发育和雄性生殖过程。
