氧化锌(ZnO)和氧化石墨烯(GO)纳米颗粒,银/锌沸石(Ag/Zn-Ze),合成了氧化石墨烯-银(GO-Ag)纳米复合材料,用X射线粉末衍射对其进行了表征,场发射扫描电子显微镜和傅里叶变换红外光谱.评估这些纳米颗粒对大肠杆菌的抗菌功效。采用摇瓶法和平板培养法进行不同浓度的培养。对于105个细胞/mL初始细菌浓度,最低抑菌浓度(MIC)分别为<160、<320、<320和>1280μg/mL,对于Ag/Zn-Ze,抑制50%细胞的抗菌浓度(IC50)为47、90、78和250μg/mL,GO,GO-Ag,和ZnO,分别。因此,摇瓶法表明,对于所有纳米粒子浓度,Ag/Zn-Ze,GO-Ag表现出更大的抑制作用,这也高度依赖于初始细菌浓度。然而,在平板培养法的情况下,Ag/Zn-Ze的抑制能力范围相似,GO-Ag,和ZnO,而GO显示较低的抑制大肠杆菌的效力。此外,当制备抗菌表面时,GO-Ag纳米复合材料比Ag/Zn-Ze表现出更高的功效。然而,Ag/Zn-Ze对Vero细胞无毒性,而GO-Ag在较高浓度下表现出严重的毒性。这项研究确立了GO-Ag和Ag/Zn-Ze作为有效的抗微生物剂;然而,在任何可能的生理相互作用的情况下,应根据GO-Ag的细胞毒性作用仔细选择它们的应用剂量。
Zinc oxide (ZnO) and graphene oxide (GO) nanoparticles, silver/zinc zeolite (Ag/Zn-Ze), and graphene oxide-silver (GO-Ag) nanocomposites were synthesized and characterized with X-ray powder Diffraction, Field Emission Scanning Electron Microscope and Fourier Transform-Infrared Spectroscopy. The antibacterial efficacy of these nanoparticles was evaluated against E. coli. by shake flask method and plate culture method for different concentrations. For 105 cells/mL initial bacterial concentration, minimum inhibitory concentration (MIC) were <160, <320, <320, and >1280 μg/mL, and antibacterial concentration at which 50% cells are inhibited (IC50) were 47, 90, 78, and 250 μg/mL for Ag/Zn-Ze, GO, GO-Ag, and ZnO, respectively. Therefore, the shake flask method showed that for all nanoparticle concentrations, Ag/Zn-Ze, and GO-Ag exhibited greater inhibition efficacy, which was also highly dependent on initial bacterial concentration. However, in case of the plate culture method, similar range of inhibition capacity was found for Ag/Zn-Ze, GO-Ag, and ZnO, whereas GO showed lower potency to inhibit E. coli. In addition, GO-Ag nanocomposite exhibited more efficacy than Ag/Zn-Ze when the antibacterial surface was prepared with those. However, Ag/Zn-Ze showed no toxicity on Vero cells, whereas GO-Ag exhibited severe toxicity at higher concentrations. This study establishes GO-Ag and Ag/Zn-Ze as potent antimicrobial agents; however, their application dosage should carefully be chosen based on cytotoxic effects of GO-Ag in case of any possible physiological interaction.