■横纹肌溶解症,可能危及生命的情况,当肌红蛋白从受损的肌肉细胞释放时,导致急性肾损伤(AKI)。α硫辛酸(ALA),一种有机硫化合物,以其抗氧化和抗炎特性而闻名,在这项研究中检查了其对大鼠横纹肌溶解诱导的AKI的潜在影响。
■六组大鼠被纳入研究,每组6只大鼠(n=6):对照组,横纹肌溶解症,用不同剂量的ALA(5、10和20mg/kg)治疗横纹肌溶解症,和单独ALA(20mg/kg)组。实验第一天肌内注射甘油诱发横纹肌溶解,而ALA连续4天腹膜内给药。肾功能参数,氧化应激标志物,并评估肾脏的组织学变化。进行蛋白质印迹分析以测量中性粒细胞明胶酶相关脂质运载蛋白(NGAL)和肿瘤坏死因子-α(TNF-α)蛋白的水平。
■血清尿素显著增加,肌酐,肾丙二醛,NGAl,在注射甘油的大鼠中观察到TNF-α蛋白水平。此外,谷胱甘肽显著减少.与横纹肌溶解组相比,ALA治疗可恢复肾脏组织学和生化异常。
■结果表明,横纹肌溶解诱导的AKI与氧化应激和炎症增加有关。ALA治疗可改善大鼠肾脏组织学异常并降低氧化应激标志物。因此,ALA可能对横纹肌溶解诱导的AKI具有潜在的保护作用。
UNASSIGNED: Rhabdomyolysis, a potentially life-threatening condition, occurs when myoglobin is released from damaged muscle cells, leading to acute kidney injury (AKI). Alpha lipoic acid (ALA), an organosulfur compound known for its anti-oxidant and anti-inflammatory properties, was examined in this study for its potential impact on rhabdomyolysis-induced AKI in rats.
UNASSIGNED: Six groups of rats were included in the study, with each group consisting of six rats (n=6): Control, rhabdomyolysis, rhabdomyolysis treated with different doses of ALA (5, 10, and 20 mg/kg), and ALA alone (20 mg/kg) groups. Rhabdomyolysis was induced by intramuscular injection of glycerol on the first day of the experiment, while ALA was administered intraperitoneally for four consecutive days. Renal function parameters, oxidative stress markers, and histological changes in the kidneys were evaluated. Western blot analysis was performed to measure the levels of neutrophil gelatinase-associated lipocalin (NGAL) and tumor necrosis factor-alpha (TNF-α) proteins.
UNASSIGNED: A significant increase in serum urea, creatinine, renal malondialdehyde, NGAl, and TNF-α protein levels was observed in glycerol-injected rats. In addition, a significant decrease in glutathione was recorded. Compared to the rhabdomyolysis group, treatment with ALA recovered kidney histological and biochemical abnormalities.
UNASSIGNED: Results suggest that rhabdomyolysis-induced AKI is associated with increased oxidative stress and inflammation. Treatment with ALA improved kidney histological abnormalities and reduced oxidative stress markers in rats. Therefore, ALA may have a potential protective effect against rhabdomyolysis-induced AKI.