地中海饮食(MD)及其生物活性成分因其神经保护特性以及影响肠道微生物群形成和代谢的能力而被提倡。通过肠道脑轴介导,微生物群的这种调节可能部分有助于MD的神经保护特性。为了探索这种潜在的相互作用,我们评估了类似地中海饮食的新型生物活性混合物(Neurosyn240)在啮齿动物慢性低度炎症模型中的神经保护特性.认知的行为测试,大脑蛋白质组学分析,16SrRNA测序,和1HNMR代谢组学分析用于了解所涉及的肠-脑轴相互作用。识别记忆,由新颖的物体识别任务(NOR)评估,对LPS损伤的反应降低,并通过补充Neurosynd240恢复。虽然开放领域的任务绩效没有达到重要意义,它与NOR表现相关,表明与这种认知变化相关的焦虑因素。与Neurosyn240相关的行为变化伴随着微生物群组成的变化,其中包括Firmicutes:拟杆菌比例的恢复和Muribaculum的增加,利肯纳尔科Alloprevotella,最值得注意的是与NOR表现显着相关的Akkermansia。Akkermansia还与代谢物5-氨基戊酸相关,苏氨酸,缬氨酸,一磷酸尿苷,和一磷酸腺苷,这反过来与NOR表现显著相关。大脑中的蛋白质组学特征受到两种干预措施的极大影响,KEGG分析强调了氧化磷酸化和神经退行性疾病相关途径的调节。有趣的是,这些蛋白质组变化的一个子集同时与Akkermansia丰度相关,主要与氧化磷酸化相关,也许暗示了一种保护性的肠-脑轴相互作用。总的来说,我们的结果表明,生物活性混合物Neurosyn240赋予认知和微生物区系复原力,以应对低度炎症的有害影响.
The Mediterranean diet (MD) and its bioactive constituents have been advocated for their neuroprotective properties along with their capacity to affect gut microbiota speciation and metabolism. Mediated through the gut brain axis, this modulation of the microbiota may partly contribute to the neuroprotective properties of the MD. To explore this potential interaction, we evaluated the neuroprotective properties of a novel bioactive blend (Neurosyn240) resembling the Mediterranean diet in a rodent model of chronic low-grade inflammation. Behavioral tests of cognition, brain proteomic analysis, 16S rRNA sequencing, and 1H NMR metabolomic analyses were employed to develop an understanding of the gut-brain axis interactions involved. Recognition memory, as assessed by the novel object recognition task (NOR), decreased in response to LPS insult and was restored with Neurosyn240 supplementation. Although the open field task performance did not reach significance, it correlated with NOR performance indicating an element of anxiety related to this cognitive change. Behavioral changes associated with Neurosyn240 were accompanied by a shift in the microbiota composition which included the restoration of the Firmicutes: Bacteroidota ratio and an increase in Muribaculum, Rikenellaceae Alloprevotella, and most notably
Akkermansia which significantly correlated with NOR performance.
Akkermansia also correlated with the metabolites 5-aminovalerate, threonine, valine, uridine monophosphate, and adenosine monophosphate, which in turn significantly correlated with NOR performance. The proteomic profile within the brain was dramatically influenced by both interventions, with KEGG analysis highlighting oxidative phosphorylation and neurodegenerative disease-related pathways to be modulated. Intriguingly, a subset of these proteomic changes simultaneously correlated with
Akkermansia abundance and predominantly related to oxidative phosphorylation, perhaps alluding to a protective gut-brain axis interaction. Collectively, our results suggest that the bioactive blend Neurosyn240 conferred cognitive and microbiota resilience in response to the deleterious effects of low-grade inflammation.