We developed a zooplankton-based water-quality evaluating method using indices of alpha diversity. Two key objectives were set: (i) the comparison of two-different quality-samples from different areas, and the verification of their differentiation, based on mesozooplankton biodiversity indices; and (ii) the development of a methodology, which was able to assess the quality of new marine water samples. Our analysis was based on a 24-year-long in situ dataset (1987-2010) of 139 samples in which 86 mesozooplankton taxa were identified. High-diversity and high evenness values were reported in the case of the \"good\" status sample, while low diversity, low evenness and high dominance values occurred at the lower quality one. A linear discriminant analysis (LDA) was conducted that discriminated the tested samples at 100%. This LDA was then used to evaluate samples of unknown quality. Finally, 90% of them were classified with a probability of correct classification (posterior probability) >95%. The present study proves that mesozooplankton diversity indices can discriminate different levels of anthropogenic impacts. In this sense, it can be used as a reliable indicator for environmental assessment in the pelagic habitats of the Mediterranean Sea.

Marine litter poses a significant environmental challenge in the Saronikos Gulf, Greece. Employing an integrated approach, data from both beach and underwater sites were analyzed. The average litter density on beaches was 2.61 items m-2, with plastic being the most common material, notably small polystyrene fragments and cigarette butts. The western part of the Gulf exhibited higher litter density, mainly due to surface circulation patterns. Most beaches fell short of meeting Good Environmental Status criteria for marine litter. Higher litter densities were observed in autumn. Benthic litter density decreased with depth, being 23 times higher in shallower waters, with plastic being the predominant type. This extensive study offers crucial insights into the pollution status and litter distribution in the Saronikos Gulf, contributing significantly to the global understanding of marine litter distribution on coastal ecosystems. Such information is crucial for raising awareness, informing policy decisions, and driving environmental actions.

Blast fishing is an illegal, ecologically destructive fishing method, fatal for many fish species at large scales. Blast fishing using dynamite is preferred by the fishers, especially small scale fisheries in Turkey, as it requires minimal effort but still results in higher catches. In the current study, demonstration of 20 controlled real-time blasting results involves species composition, size of fish, catch rates, catch composition, commercial/discard ratios, CPUE estimates and clinical observations of external and internal abnormality symptoms of fish species. Blasting trials were carried out with special permission in limited numbers because of its harmful effect. The collected data was tested using PRIMER v6 and diversity indices were also analysed. For the similarity between collected species cluster analysis was used to examine the stability of the results. Multidimensional scaling (MDS) was also applied for the assessment of fish species in the catch. A total of 1014 individuals (63.8 kg) were collected and 18 fish species belonging to seven families were examined. According to laboratory examinations, it was found that blasting caused different external and internal abnormality symptoms in the sampled fish species during the study.

Hyrographic and trophic gradient along Izmir Bay used to assess their effects on the copepod composition and diversity. Trophic state of the bay varied from oligotrophic in the outer region to hypertrophic condition in the inner region as stated by trophic index (TRIX). Aegean Sea water entrance and trophic conditions of the bay affected species assemblages. Oithona davisae, Oithona nana and Acartia clausi were associated with hyper-eutrophic conditions whereas the typical Aegean Sea species, such as Oncaea media group, Oithona similis, Paracalanus parvus, A. clausi, Farranula rostrata and Calocalanus styliremis were related to oligotrophic condition. Copepod diversity indices decreased linearly along the trophic gradient, from oligotrophic to hyper-eutrophic conditions, highlighting the substantial impact of eutrophication on the copepod community structure in the inner region. These findings emphasize TRIX\'s value in assessing eutrophication in anthropized systems, and immense potential for using copepod composition and diversity in monitoring programs for ecological assessments studies.

Bays are vulnerable ecosystems generally located near densely populated areas where toxic metals tend to accumulate and stay longer, affecting marine life. This study aimed to investigate the age-based health risks arising from Hg, Cd, Pb, and As in demersal fish captured from two major bays in the Aegean Sea. For this purpose, red mullet, whiting, piper gurnard, and tub gurnard, frequently consumed species, were caught from Saros and Edremit Bays. Toxic metal concentrations were determined from the muscle tissue of fish. Health risk assessments were conducted by the estimation of weekly intake (EWI), provisional tolerable weekly intake (PTWI), target hazard quotient (THQ), total THQ (TTHQ), and target carcinogenic risk (TR). Red mullet from Edremit Bay was the species with the highest toxic metal levels, which were 1.597 mg/kg, 0.041 mg/kg, 0.070 mg/kg, and 19.351 mg/kg for Hg, Cd, Pb, and As, respectively. Whiting from Edremit Bay had higher mean concentrations of Hg and As than those from Saros Bay. The levels of Hg, Pb, and As (0.328, 0.043, and 0.574 mg/kg) in the tub gurnard were higher in comparison with the piper gurnard (0.252, 0.020, and 0.382 mg/kg) caught in the same station in Saros. TTHQs of red mullet and whiting from the same bay were found to be > 1, indicating potential health risks for all nine age categories studied. On the other hand, TTHQs of all species from Saros Bay were determined to be > 1 for the first four age categories, which might trigger health risks for children and adolescents. According to the TR index for Pb, no risk was determined for the fish from both bays. However, TR calculations for inorganic As indicated high cancer risk in most of the age categories for red mullet and whiting from Edremit Bay. To sum up, the results revealed that the fish captured from Edremit Bay posed serious health risks in terms of Hg and As concentrations for all nine age categories. Surveillance and monitoring of toxic metal levels in demersal fish and population-based health risk evaluation are vital in heavily populated bays.

Microplastics (MPs) and their impacts have been extensively studied in the Mediterranean region. However, more research has yet to be conducted on assessing the extent of microplastic (MP) pollution in the eastern Aegean Sea, specifically in Izmir Bay. This study aims to evaluate the current state of MP pollution in surface water and sediment samples collected from Izmir Bay. Ten sampling stations were specifically selected, including locations near stream discharge points, maritime transportation piers, and port areas. Surface water samples were collected using a manta trawl net, while sediment samples were obtained using a Van Veen grab. The mean MP abundances in surface water ranged from 1,083,882 to 8,091,684 items/km2. Fragment type MPs were dominant. In terms of size category, it was found that MP s of 500µm size were dominant. The dominant color of MPs was white. ATR-FTIR analyses revealed that polyethylene and polypropylene were the dominant polymer types. MP concentrations in sediment ranged from 2,125 to 4,925 items/m2, with fiber-type MPs being the most abundant. Black-colored MPs were found to dominate in sediment samples. Overall, the MP levels in Izmir Bay were higher than previous studies findings. Therefore, it is crucial to conduct long-term monitoring studies to obtain more consistent and reliable data on MP pollution levels in Izmir Bay.

Physiological stress patterns of marine organisms in their natural habitats are considerably complex in space and time. These patterns can eventually contribute in the shaping of fish\' thermal limits under natural conditions. In the view of the knowledge gap regarding red porgy\'s thermal physiology, in combination with the characterization of the Mediterranean Sea as a climate change \'\'hotspot\'\', the aim of the present study was to investigate this species biochemical responses to constantly changing field conditions. To achieve this goal, Heat Shock Response (HSR), MAPKs pathway, autophagy, apoptosis, lipid peroxidation and antioxidant defense were estimated and exhibited a seasonal pattern. In general, all the examined biochemical indicators expressed high levels parallel to the increasing seawater temperature in spring, although several bio-indicators have shown increased levels when fish were cold-acclimatized. Similar to other sparids, the observed patterns of physiological responses in red porgy may support the concept of eurythermy.

Macroalgae exhibit beneficial bioactivities for human health. Thus, the aim of the present study was to examine the antioxidant and anticancer potential of 14 macroalgae species\' extracts, namely, Gigartina pistillata, Gigartina teedei, Gracilaria gracilis, Gracilaria sp., Gracilaria bursa pastoris, Colpomenia sinuosa, Cystoseira amentacea, Cystoseira barbata, Cystoseira compressa, Sargassum vulgare, Padina pavonica, Codium fragile, Ulva intestinalis, and Ulva rigida, from the Aegean Sea, Greece. The antioxidant activity was assessed using DPPH, ABTS•+, •OH, and O2•- radicals\' scavenging assays, reducing power (RP), and protection from ROO•-induced DNA plasmid damage assays. Moreover, macroalgae extracts\' total polyphenol contents (TPCs) were assessed. Extracts\' inhibition against liver HepG2 cancer cell growth was assessed using the XTT assay. The results showed that G. teedei extract\'s IC50 was the lowest in DPPH (0.31 ± 0.006 mg/mL), ABTS•+ (0.02 ± 0.001 mg/mL), •OH (0.10 ± 0.007 mg/mL), O2•- (0.05 ± 0.003 mg/mL), and DNA plasmid breakage (0.038 ± 0.002 mg/mL) and exhibited the highest RP (RP0.5AU 0.24 ± 0.019 mg/mL) and TPC (12.53 ± 0.88 mg GAE/g dw). There was also a significant correlation between antioxidant activity and TPC. P. pavonica (IC50 0.93 ± 0.006 mg/mL) exhibited the highest inhibition against HepG2 cell growth. Conclusively, some of the tested extracts exhibited significant chemopreventive properties, and so they may be used for food products.

The feeding habits of organisms are important elements in their ecological role and are affected by several factors. The present study provides for the first time information on the diet and feeding strategy of Dentex maroccanus (Valenciennes, 1830) and examines the effects of various factors on the species\' feeding activity. Various indices (vacuity index, numerical and weight proportion, frequency of occurrence, alimentary coefficient, index of relative importance, diet breadth and overlap, Shannon-Wiener index, and trophic level) were estimated. The diet of the species consisted of 18 different prey taxa. The most important prey taxon was Decapoda. The study of the feeding strategy showed the narrow width of the species. Body size was found to significantly affect the species\' feeding habits. Polychaeta and Stomatopoda were found only in individuals with size ≥165 mm, Bivalvia mainly in sizes ≤120 mm, and Decapoda in the intermediate sizes. The largest individuals showed the lowest overlap with all other size groups. The trophic level increased from 3.7 in young individuals to 4.0 in larger sizes, indicating the carnivorous character of the species. The results of the present work contribute to a better knowledge of the species\' feeding ecology.

European marine waters are infested with plastic, with an average density of 1 plastic item in every four square-meters. Research relevant to MPs-NPs ingestion by wild decapods in European waters is limited, none of which regards the European spiny lobster. Totally 4102 plastic particles were extracted from the spiny lobster stomach tissues of both sexes. Out of the 63 samples analysed only three (4.8%) of them were found with no plastic particles. The range of number of MPs in stomachs was from 20 to 273 MPs individual-1. The 98.3% were fragments. In total 3833 plastic particles were extracted from the gill tissues of both sexes. MPs were found in all samples (n = 50),99.2% of the detected particles were fragments. The MPs detected in gills ranged from 11 to 339 MPs individual-1. The DLS method was used in order to evaluate the NPs presence. Nanoplastics were detected in 22.6% of stomachs and in the 48.1% of gills. A total of 43 polymer types were identified in both tissues. Also, our study assessed the accumulation of heavy metals at the edible tail muscle. Certain elements were detected above the EU\'s Maximum Residue Level, including arsenic. The present results are alarming and the potential human health implications could be serious.