背景:肌细胞分化是为了收缩功能,在身体代谢和能量止血中起着重要作用,通过不同的代谢途径,如葡萄糖和蛋白质代谢途径。丙氨酸氨基转移酶(ALT)通过可逆地催化丙氨酸和α-酮戊二酸之间的氨基转移以形成丙酮酸和谷氨酸,并通过介导这四种主要中间代谢物的转化而发挥关键作用。ALT在禁食和长时间运动期间对能量稳态起着重要作用,当肌肉蛋白质必须首先被分解成其组成氨基酸时。
方法:小鼠骨骼成肌细胞系C2C12在Dulbecco改良的鹰培养基(DMEM)生长培养基中培养,提供2%马血清,补充1uM胰岛素,2mM谷氨酰胺和青霉素和链霉素抗生素7天。每24小时更新分化培养基。然后,用胰岛素和地塞米松处理C2C12细胞以检查它们对肌细胞ALT活性的影响。
结果:在我们的研究中,我们发现在不同的影响下对ALT活性的影响,包括C2C12分化,地塞米松和胰岛素治疗,揭示了ALT活动之间的动态相互作用,丙氨酸代谢,和蜂窝状态,比如分化和应激反应。
结论:该研究为C2C12细胞在分化和药物治疗过程中ALT活性和丙氨酸代谢的动态调节提供了有价值的见解。鼓励进一步研究探索潜在的机制及其对肌肉功能的影响,代谢紊乱的分化和潜在的治疗干预。
BACKGROUND: The muscle cells myocytes are differentiated for the purpose of contraction function, which plays a major role in body metabolism and energy haemostasis, through different metabolic pathways, such as glucose and protein metabolic pathways. Alanine aminotransferase (
ALT) plays a crucial role by reversibly catalysing transamination between alanine and a-ketoglutarate to form pyruvate and glutamate and by mediating the conversion of these four major intermediate metabolites.
ALT plays important roles for energy homeostasis during fasting and prolonged exercise anaerobically, when muscle protein must first be broken down into its constituent amino acids.
METHODS: Mouse skeletal myoblast cell line C2C12 was cultured in Dulbecco\'s modified eagle medium (DMEM) growth medium, supplied with 2% horse serum supplemented with 1 uM insulin, 2 mM glutamine and penicillin and streptomycin antibiotics for seven days. The differentiation medium is refreshed every 24 hours. Then, C2C12 cells were treated with insulin and dexamethasone to examine their effects on myocytes\'
ALT activity.
RESULTS: In our study, we found an impact on
ALT activity under different influences, including C2C12 differentiation, dexamethasone and insulin treatments, which shed light on the dynamic interplay between ALT activity, alanine metabolism, and cellular states, like differentiation and stress responses.
CONCLUSIONS: The study provides valuable insights into the dynamic regulation of
ALT activity and alanine metabolism in C2C12 cells across differentiation and drug treatments. Further research is encouraged to explore the underlying mechanisms and their implications for muscle function, differentiation and potential therapeutic interventions in metabolic disorders.