未经批准:随着牙周炎的进展,口腔微生物群落动态变化。在这项研究中,我们在患有III期牙周炎的年轻男性中评估了优势细菌及其在潜在途径中的作用.
UNASSIGNED:进行16SrRNA测序以评估患有I期和III期牙周炎的男性之间口腔细菌组成的变化,并确定每组的优势细菌。基于16SrRNA测序数据获得功能预测。III期牙周炎主要途径的抑制剂用于研究体内和体外牙周炎中主要细菌的作用。
未经评估:Chao1指数,观察到的物种和系统发育多样性(PD)全树值在Ⅲ期牙周炎组中显著高于Ⅲ期牙周炎组。β-多样性表明样本可以根据牙周炎的分期进行划分。Ⅲ期牙周炎的优势菌为普雷沃氏菌,Prevotella_7和Dialister,而在I期牙周炎中,是心脏细菌。KEGG分析预测口腔微生物组的变化可能与NOD样受体信号通路有关。这个通路的抑制剂,NOD-IN-1,减少中间假单胞菌诱导的Tnf-αmRNA表达和增加中间假单胞菌诱导的IL-6mRNA表达,与ELISA结果一致。免疫组织化学证实NOD-IN-1在中间型牙周炎中下调TNF-α和IL-6的表达。
UNASSIGNED:年轻男性口腔细菌的组成根据牙周炎的分期而变化。III期牙周炎的年轻男性的口腔小耳物种丰富度高于I期牙周炎。Prevotella是III期牙周炎年轻男性的优势细菌,而抑制NOD样受体信号通路可以减轻中间疟原虫引起的牙周炎症。
UNASSIGNED: As periodontitis progresses, the oral microbiota community changes dynamically. In this study, we evaluated the dominant bacteria and their roles in the potential pathway in young males with stage III periodontitis.
UNASSIGNED: 16S rRNA sequencing was performed to evaluate variations in the composition of oral bacteria between males with stage I and III periodontitis and identify the dominant bacteria of each group. Function prediction was obtained based on 16S rRNA sequencing data. The inhibitor of the predominant pathway for stage III periodontitis was used to investigate the role of the dominant bacteria in periodontitis in vivo and in vitro.
UNASSIGNED: Chao1 index, Observed Species and Phylogenetic Diversity (PD) whole tree values were significantly higher in the stage III periodontitis group. β-diversity suggested that samples could be divided according to the stages of periodontitis. The dominant bacteria in stage III periodontitis were Prevotella, Prevotella_7, and Dialister, whereas that in stage I periodontitis was Cardiobacterium. KEGG analysis predicted that variations in the oral microbiome may be related to the NOD-like receptor signaling pathway. The inhibitor of this pathway, NOD-IN-1, decreased P. intermedia -induced Tnf-α mRNA expression and increased P. intermedia -induced Il-6 mRNA expression, consistent with the ELISA results. Immunohistochemistry confirmed the down-regulation of TNF-α and IL-6 expressions by NOD-IN-1 in P. intermedia-induced periodontitis.
UNASSIGNED: The composition of the oral bacteria in young males varied according to the stage of periodontitis. The species richness of oral microtia was greater in young males with stage III periodontitis than those with stage I periodontitis. Prevotella was the dominant bacteria in young males with stage III periodontitis, and inhibition of the NOD-like receptor signaling pathway can decrease the periodontal inflammation induced by P. intermedia.