Abstract：
OBJECTIVE: The aim was to isolate a neotype bifidobacteria strain and evaluate its in vitro probiotic potential.
RESULTS: Bifidobacterium pseudolongum YY-26 (CGMCC 24310) was isolated from faeces of mice treated with low-molecular-weight hydrolyzed guar gum (GMPS) and identified based on 16S rRNA sequence and genome sequence. Whole-genome sequencing obtained using PacBio\'s single-molecular and Illumina\'s paired-end sequencing technology. A genome of 2.1 Mb in length, with 1877 predicted protein-coding sequences was obtained. Carbohydrate-Activity enZyme analysis revealed that YY-26 encodes 66 enzymes related to carbohydrate metabolism. Whole genome sequence analysis revealed the typical probiotic characteristics of YY-26, including safety in genetic level and ability to produce beneficial metabolites and extracellular polysaccharides. Ability of extensive carbon source utilization and short-chain fatty acid production was observed with single YY-26 cultivation. Considerable acetic acids and lactic acids were determined in GMPS utilization. YY-26 showed tolerance to simulated gastrointestinal tract and displayed appreciable antioxidant activity of free radical scavenging.
CONCLUSIONS: B. pseudolongum YY-26 was identified with numerous probiotic-associated genes and its probiotic characteristics were verified in vitro.
CONCLUSIONS: This study supplemented with limited publicly information regarding the genomes of B. pseudolongum strains and revealed the probiotic potential of YY-26.
RESULTS: Bifidobacterium pseudolongum YY-26 (CGMCC 24310) was isolated from faeces of mice treated with low-molecular-weight hydrolyzed guar gum (GMPS) and identified based on 16S rRNA sequence and genome sequence. Whole-genome sequencing obtained using PacBio\'s single-molecular and Illumina\'s paired-end sequencing technology. A genome of 2.1 Mb in length, with 1877 predicted protein-coding sequences was obtained. Carbohydrate-Activity enZyme analysis revealed that YY-26 encodes 66 enzymes related to carbohydrate metabolism. Whole genome sequence analysis revealed the typical probiotic characteristics of YY-26, including safety in genetic level and ability to produce beneficial metabolites and extracellular polysaccharides. Ability of extensive carbon source utilization and short-chain fatty acid production was observed with single YY-26 cultivation. Considerable acetic acids and lactic acids were determined in GMPS utilization. YY-26 showed tolerance to simulated gastrointestinal tract and displayed appreciable antioxidant activity of free radical scavenging.
CONCLUSIONS: B. pseudolongum YY-26 was identified with numerous probiotic-associated genes and its probiotic characteristics were verified in vitro.
CONCLUSIONS: This study supplemented with limited publicly information regarding the genomes of B. pseudolongum strains and revealed the probiotic potential of YY-26.
摘要：
目的：目的是分离新型双歧杆菌菌株并评估其体外益生菌潜力。
结果：从用低分子量水解瓜尔胶（GMPS）处理的小鼠粪便中分离出假双歧杆菌YY-26（CGMCC24310），并根据16SrRNA序列和基因组序列进行鉴定。使用PacBio的单分子和Illumina的配对末端测序技术获得的全基因组测序。基因组长度为2.1Mb，获得了1877个预测的蛋白质编码序列。碳水化合物活性酶分析显示YY-26编码66种与碳水化合物代谢相关的酶。全基因组序列分析揭示了YY-26的典型益生菌特征，包括遗传水平的安全性和产生有益代谢物和胞外多糖的能力。用一次YY-26培养观察到广泛的碳源利用和短链脂肪酸生产的能力。在GMPS利用率中确定了相当多的乙酸和乳酸。YY-26对模拟胃肠道表现出耐受性，并表现出明显的自由基清除抗氧化活性。
结论：B.假龙骨YY-26被鉴定为具有许多益生菌相关基因，并在体外验证了其益生菌特性。
结论：本研究补充了有限的关于假龙骨芽孢杆菌菌株基因组的公开信息，并揭示了YY-26的益生菌潜力。
结果：从用低分子量水解瓜尔胶（GMPS）处理的小鼠粪便中分离出假双歧杆菌YY-26（CGMCC24310），并根据16SrRNA序列和基因组序列进行鉴定。使用PacBio的单分子和Illumina的配对末端测序技术获得的全基因组测序。基因组长度为2.1Mb，获得了1877个预测的蛋白质编码序列。碳水化合物活性酶分析显示YY-26编码66种与碳水化合物代谢相关的酶。全基因组序列分析揭示了YY-26的典型益生菌特征，包括遗传水平的安全性和产生有益代谢物和胞外多糖的能力。用一次YY-26培养观察到广泛的碳源利用和短链脂肪酸生产的能力。在GMPS利用率中确定了相当多的乙酸和乳酸。YY-26对模拟胃肠道表现出耐受性，并表现出明显的自由基清除抗氧化活性。
结论：B.假龙骨YY-26被鉴定为具有许多益生菌相关基因，并在体外验证了其益生菌特性。
结论：本研究补充了有限的关于假龙骨芽孢杆菌菌株基因组的公开信息，并揭示了YY-26的益生菌潜力。
